Department of Biochemistry and Biotechnology, KNUST, Kumasi, Ghana; Department of Science Laboratory Technology, Federal Polytechnic Ede, Nigeria.
Department of Biochemistry and Biotechnology, KNUST, Kumasi, Ghana.
J Ethnopharmacol. 2022 Feb 10;284:114768. doi: 10.1016/j.jep.2021.114768. Epub 2021 Oct 21.
Hura crepitans is commonly used to treat liver diseases in Nigeria and Ghana. Previous studies have supported its ethnomedicinal use in protecting the liver. The present study aimed at assessing the effect of H. crepitans stem bark on the subacute carbon tetrachloride (CCl)-induced liver damage in rats.
The protective activities of ethanolic extract of H. crepitans stem bark was evaluated in CCl-induced subacute liver damage in rats (1:1 v/v in olive oil, intraperitoneally (i.p.), twice weekly for 8 weeks). Blood samples were obtained from the rats and used for some biochemical analysis such as liver function test (Aspartate transaminase, AST; Alanine aminotransferase, ALT; and Alkaline phosphatase, ALP), liver fibrotic indices (Aspartate platelet ratio index, APRI; AST/ALT and AST/PLT ratios) and oxidative stress markers (Malondialdehyde, MDA; Reduced glutathione, GSH; Glutathione S-transferase, GST; Glutathione peroxidase, GPx; and superoxide dismutase, SOD). Histopathological analyses were carried out to determine the expression of pro-inflammatory (NF-κB, COX-2, IL-17 and IL-23) using immunohistochemical techniques.
Oral administration of H. crepitans to CCl-induced hepatic injured rats significantly decreased oxidative stress, increased the levels of SOD, GSH, GST and GPx with reduced MDA levels. The plant also mitigated liver injury as evidenced in the significantly reduced levels of AST, ALT and ALP, while it inhibited the inflammatory process via the inhibition of NF-κB, and consequently down-regulateed the pro-inflammatory cytokines COX-2, IL-17 and IL-23, respectively. Biochemical observations were supported by improvement in liver microarchitecture.
The Hura crepitans demonstrated antioxidant, antiinflammatory and antifibrotic effect in hepatic injured rats. The study in a way justifies the traditional use of the plant for the treatment of subacute liver diseases in Nigerian Traditional medicine.
在尼日利亚和加纳，猴欢喜通常被用于治疗肝脏疾病。先前的研究支持其在肝脏保护方面的民间医学用途。本研究旨在评估猴欢喜茎皮对大鼠亚急性四氯化碳（CCl）诱导的肝损伤的影响。
用橄榄油（1:1，v/v）腹腔内（i.p.）每周两次，共 8 周，评价猴欢喜茎皮乙醇提取物对 CCl 诱导的亚急性肝损伤的保护活性。从大鼠中采集血液样本，用于一些生化分析，如肝功能测试（天门冬氨酸转氨酶，AST；丙氨酸氨基转移酶，ALT；碱性磷酸酶，ALP）、肝纤维化指数（天冬氨酸血小板比指数，APRI；AST/ALT 和 AST/PLT 比值）和氧化应激标志物（丙二醛，MDA；还原型谷胱甘肽，GSH；谷胱甘肽 S-转移酶，GST；谷胱甘肽过氧化物酶，GPx；超氧化物歧化酶，SOD）。通过免疫组织化学技术，进行组织病理学分析以确定促炎（NF-κB、COX-2、IL-17 和 IL-23）的表达。
给予 CCl 诱导的肝损伤大鼠口服猴欢喜可显著降低氧化应激，增加 SOD、GSH、GST 和 GPx 的水平，降低 MDA 水平。该植物还减轻了肝损伤，表现为 AST、ALT 和 ALP 水平显著降低，同时通过抑制 NF-κB 抑制炎症过程，从而分别下调促炎细胞因子 COX-2、IL-17 和 IL-23。生化观察结果得到了改善肝微结构的支持。
猴欢喜在肝损伤大鼠中表现出抗氧化、抗炎和抗纤维化作用。该研究在一定程度上证明了该植物在尼日利亚传统医学中用于治疗亚急性肝病的传统用途。
