Devi Thiyam B, Jena Sarita, Patra Biswajit, Singh Kabrambam D, Chawla Saurabh, Raina Vishakha, Koijam Arunkumar Singh, Parida Ajay, Rajashekar Yallappa
Insect Bioresource Laboratory, Animal Bioresources Programme, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Govt. of India, Takyelpat, Manipur, India.
School of Biotechnology, Kalinga Institute of Industrial Technology, Deemed to be University, Bhubaneswar, Odisha, India.
Front Pharmacol. 2022 Nov 23;13:1055765. doi: 10.3389/fphar.2022.1055765. eCollection 2022.
In present study, the acute and sub-acute toxicities of Dihydro--coumaric acid isolated from the leaves of (Hemsl.) A. Gray was studied for safety issues in mammals For acute toxicity tests, isolated compound was administered orally in both male and female BALB/c mice at the doses of 200, 800, and 1,600 mg/kg body weight for 7 days. In sub-acute toxicity study 50 and 500 mg/kg bw of the compound was orally administered for 14 days. Toxicity induced behavioural changes, haematological parameters, biochemical markers and histopathological sections were studied after Dihydro--coumaric acid administration. The vital organs like heart, kidney, uterus and testis revealed no adverse effects at doses of upto 1,600 mg/kg bw and 500 mg/kg bw. Slight hepatotoxicity was however demonstrated by ALT and AST assay but histopathological section did not concur as much. The study demonstrated insignificant difference in the percentage of feed intake, water intake, weight gain, haematological parameters and histopathological changes, with no toxicity signs and mortality. Dihydro--coumaric acid can be regarded as safe in both acute and sub-acute toxicity assay in both sexes. This indicates Dihydro--coumaric acid as a viable alternative to synthetic pesticides.
在本研究中，为研究哺乳动物的安全性问题，对从（Hemsl.）A. Gray叶片中分离出的二氢香豆酸的急性和亚急性毒性进行了研究。在急性毒性试验中，将分离出的化合物以200、800和1600毫克/千克体重的剂量口服给予雄性和雌性BALB/c小鼠，持续7天。在亚急性毒性研究中，以50和500毫克/千克体重的剂量口服给予该化合物14天。在给予二氢香豆酸后，研究了毒性引起的行为变化、血液学参数、生化标志物和组织病理学切片。心脏、肾脏、子宫和睾丸等重要器官在高达1600毫克/千克体重和500毫克/千克体重的剂量下未显示出不良反应。然而，谷丙转氨酶（ALT）和谷草转氨酶（AST）测定显示有轻微肝毒性，但组织病理学切片结果不太一致。该研究表明，在采食量、饮水量、体重增加、血液学参数和组织病理学变化百分比方面差异不显著，无毒性迹象和死亡情况。二氢香豆酸在两性的急性和亚急性毒性试验中均可视为安全。这表明二氢香豆酸是合成农药的一种可行替代品。
