State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo 315211, China; Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo 315211, China; Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China.
Comp Biochem Physiol B Biochem Mol Biol. 2022 Feb-Mar;258:110680. doi: 10.1016/j.cbpb.2021.110680. Epub 2021 Oct 21.
Osteoprotegerin (OPG) is a member of the tumor necrosis factor receptor superfamily, contributing to inflammation, apoptosis, and differentiation. However, the function of OPG in the host immune system of teleosts remains unclear. Here, we cloned the cDNA of the LcOPG gene from large yellow croaker. LcOPG mRNA was expressed in all analyzed tissues and was upregulated by Vibrio alginolyticus infection in immune tissues and monocytes/macrophages (MO/MФ). Subsequently, the LcOPG protein was expressed and purified using a prokaryotic expression system. Recombinant LcOPG protein (rLcOPG) treatment suppressed V. alginolyticus-induced pro-inflammatory cytokine and enhanced V. alginolyticus-induced anti-inflammatory cytokine mRNA expression. Furthermore, rLcOPG decreased V. alginolyticus-induced MO/MФ apoptosis. Therefore, the results indicate that LcOPG might play a role in the immune response of V. alginolyticus-infected large yellow croaker.
骨保护素（OPG）是肿瘤坏死因子受体超家族的一员，参与炎症、细胞凋亡和分化。然而，OPG 在硬骨鱼类宿主免疫系统中的功能尚不清楚。本研究从大黄鱼中克隆了 LcOPG 基因的 cDNA。LcOPG mRNA 在所有分析的组织中均有表达，在免疫组织和单核细胞/巨噬细胞（MO/MФ）中受到弧菌感染的上调。随后，使用原核表达系统表达和纯化了 LcOPG 蛋白。重组 LcOPG 蛋白（rLcOPG）处理抑制了弧菌诱导的促炎细胞因子，并增强了弧菌诱导的抗炎细胞因子 mRNA 的表达。此外，rLcOPG 减少了弧菌诱导的 MO/MФ 细胞凋亡。因此，这些结果表明 LcOPG 可能在大黄鱼弧菌感染的免疫反应中发挥作用。
