Liao J W, Ma P, Yang H H, Pan J Y
Department of Psychiatry, the First Affiliated Hospital of Jinan University,Guangzhou 510632,China.
Sleep Medicine Center,the First Affiliated Hospital of Jinan University,Guangzhou 510632,China.
Zhonghua Yi Xue Za Zhi. 2021 Oct 26;101(39):3227-3231. doi: 10.3760/cma.j.cn112137-20210306-00573.
To investigate the characteristics of glutamatergic neurotransmitter and neurotransmission dysfunction in patients with major depressive disorder (MDD) and its relationship with clinical characteristics. Fifty-two patients with MDD and 51 healthy controls were selected. Hamilton Depression scale-17 (HAMD-17) and Hamilton Anxiety Scale (HAMA) were used to evaluate the symptoms of depression and anxiety. The serum glutamate level was measured by enzyme-linked immunosorbent assay (ELISA). Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the mRNA level of NR1 subunit of NMDA receptor. There was no significant difference in gender and age between MDD group and healthy control group. Compared with the control group, the level of serum glutamate [(35±6) mg/L vs (29±6) mg/L, = 0.021] and mRNA level of NR1 (1.5±0.8 vs 0.8±0.6, = 0.001) in MDD group were significantly higher. In MDD group, serum glutamate level was positively correlated with depression core symptom score ( = 0.52, = 0.028), and mRNA level of NR1 subunit was positively correlated with the total course of disease ( = 0.42, = 0.024). MDD patients may have disorder of glutamatergic neurotransmitter and abnormal expression of NR1 subunit.
探讨重度抑郁症（MDD）患者谷氨酸能神经递质及神经传递功能障碍的特点及其与临床特征的关系。选取52例MDD患者和51例健康对照者。采用汉密尔顿抑郁量表17项（HAMD-17）和汉密尔顿焦虑量表（HAMA）评估抑郁和焦虑症状。采用酶联免疫吸附测定（ELISA）法检测血清谷氨酸水平。采用逆转录聚合酶链反应（RT-PCR）检测N-甲基-D-天冬氨酸受体（NMDA受体）NR1亚基的mRNA水平。MDD组与健康对照组在性别和年龄上无显著差异。与对照组相比，MDD组血清谷氨酸水平[（35±6）mg/L对（29±6）mg/L，P = 0.021]和NR1的mRNA水平（1.5±0.8对0.8±0.6，P = 0.001）显著升高。在MDD组中，血清谷氨酸水平与抑郁核心症状评分呈正相关（r = 0.52，P = 0.028），NR1亚基的mRNA水平与病程总长度呈正相关（r = 0.42，P = 0.024）。MDD患者可能存在谷氨酸能神经递质紊乱及NR1亚基表达异常。
