利用DNA测序对植物线粒体或叶绿体特定位点的RNA编辑进行定量分析。
Quantitative Analysis of RNA Editing at Specific Sites in Plant Mitochondria or Chloroplasts Using DNA Sequencing.
作者信息
Yang Yang, Shan Weixing
机构信息
State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China.
出版信息
Bio Protoc. 2021 Sep 20;11(18):e4154. doi: 10.21769/BioProtoc.4154.
Cytidine-to-uridine (C-to-U) RNA editing is one of the most important post-transcriptional RNA processing in plant mitochondria and chloroplasts. Several techniques have been developed to detect the RNA editing efficiency in plant mitochondria and chloroplasts, such as poisoned primer extension (PPE) assays, high-resolution melting (HRM) analysis, and DNA sequencing. Here, we describe a method for the quantitative detection of RNA editing at specific sites by sequencing cDNA from plant leaves to further evaluate the effect of different treatments or plant mutants on the C to U RNA editing in mitochondria and chloroplasts.
胞嘧啶到尿嘧啶（C-to-U）RNA编辑是植物线粒体和叶绿体中最重要的转录后RNA加工过程之一。已经开发了几种技术来检测植物线粒体和叶绿体中的RNA编辑效率，如中毒引物延伸（PPE）分析、高分辨率熔解（HRM）分析和DNA测序。在这里，我们描述了一种通过对植物叶片cDNA进行测序来定量检测特定位点RNA编辑的方法，以进一步评估不同处理或植物突变体对线粒体和叶绿体中C到U RNA编辑的影响。
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