Institute of Food and Nutritional Sciences, PMAS-Arid Agriculture University, Rawalpindi, Pakistan.
IET Nanobiotechnol. 2021 May;15(3):309-317. doi: 10.1049/nbt2.12014. Epub 2021 Feb 10.
Co-surfactant free l-ascorbic acid (LAA) nanoemulsions were prepared using mixed surfactants (Soya lecithin and Tween 80). Response surface methodology (RSM) was used to optimise the emulsifying conditions for LAA nanoemulsions. The effects of water proportion (6%-14% w/w), homogenisation pressure (80-160 MPa), surfactant concentrations (4%-12% w/w) and LAA concentration (0.5-1.3 w/w) on responses (size of droplets and nanoemulsion stability) were investigated. RSM results showed that the values of responses can be successfully predicted through second-order polynomial model. The coefficients of determinations for droplet size and nanoemulsion stability were 0.9375 and 0.9027, respectively. The optimum preparation conditions for l-LAA nanoemulsion were 9.04% water proportion, 114.48 MPa homogenisation pressure, 7.36% surfactant concentration and 1.09% LAA concentration. At the end of one month storage study, the retention of LAA in optimised nanoemulsions stored at 4°C and 25°C were 74.4% and 66.7%, respectively. These results may provide valuable contributions for food and pharmaceutical industry to develop delivery system for food additives and nutraceutical components.
无辅助表面活性剂的 l-抗坏血酸（LAA）纳米乳剂是使用混合表面活性剂（大豆卵磷脂和吐温 80）制备的。响应面法（RSM）用于优化 LAA 纳米乳剂的乳化条件。考察了水相比例（6%-14%w/w）、均质压力（80-160MPa）、表面活性剂浓度（4%-12%w/w）和 LAA 浓度（0.5-1.3w/w）对响应值（液滴大小和纳米乳稳定性）的影响。RSM 结果表明，通过二次多项式模型可以成功预测响应值。液滴大小和纳米乳稳定性的决定系数分别为 0.9375 和 0.9027。l-LAA 纳米乳的最佳制备条件为水相比例 9.04%、均质压力 114.48MPa、表面活性剂浓度 7.36%和 LAA 浓度 1.09%。在一个月的储存研究结束时，在 4°C 和 25°C 下储存的优化纳米乳液中 LAA 的保留率分别为 74.4%和 66.7%。这些结果可能为食品和制药行业开发食品添加剂和营养成分的传递系统提供有价值的贡献。
