用于在Wistar大鼠舌石蜡包埋切片中成像白色念珠菌的RNA适体探针的验证
Validation of RNA Aptamer Probes to Image Candida albicans in Paraffin-Embedded Sections of Wistar Rat Tongue.
作者信息
Bachtiar Boy M, Srisawat Chatchawan, Rahayu Retno Pudji, Soedjodono Retno D, Prabandari Silvia Arin, Bachtiar Endang W
机构信息
Department of Oral Biology and Oral Science Research Center, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
Department of Biochemistry and NANOTEC-Mahidol, University Center of Excellence in Nanotechnology for Cancer Diagnosis and Treatment, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok Noi, Bangkok, Thailand.
出版信息
Eur J Dent. 2022 Jul;16(3):543-548. doi: 10.1055/s-0041-1735794. Epub 2021 Oct 25.
OBJECTIVE
This study aimed to validate the use of Ca-apt-1, an RNA aptamer, that we generated previously as a probe for immunostaining of in rat tongue paraffin-fixed tissue sections MATERIAL AND METHODS: The performance of Ca-apt-1 as a detector molecule was compared with that of anti- polyclonal antibody (PcAb), which was used as a positive control. Immunostaining images were visualized by light microscopy and were analyzed by using ImageJ software.
RESULTS
Microscopic results demonstrated that Ca-apt-1 specifically recognized and immunostained cells of rat tongue candidiasis, with a specificity comparable to that of PcAb. ImageJ analysis showed that the area (pixels) detected by Ca-apt-1 was wider than that detected by the antibody. This indicates that the binding affinity of Ca-apt-1 toward was better than that of PcAb on paraffin-embedded tissues.
CONCLUSION
This study demonstrates that Ca-apt-1 can be used as a probe for immunostaining of fixed tissue sections for oral candidiasis diagnosis.
目的
本研究旨在验证我们之前生成的RNA适配体Ca-apt-1作为大鼠舌石蜡固定组织切片中免疫染色探针的用途。材料与方法：将Ca-apt-1作为检测分子的性能与用作阳性对照的抗多克隆抗体（PcAb）进行比较。通过光学显微镜观察免疫染色图像，并使用ImageJ软件进行分析。
结果
显微镜结果表明，Ca-apt-1能特异性识别并免疫染色大鼠舌念珠菌病的细胞，其特异性与PcAb相当。ImageJ分析显示，Ca-apt-1检测到的面积（像素）比抗体检测到的更宽。这表明在石蜡包埋组织上，Ca-apt-1与的结合亲和力优于PcAb。
结论
本研究表明，Ca-apt-1可作为固定组织切片免疫染色的探针用于口腔念珠菌病诊断。
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