Lau L F, Nathans D
Proc Natl Acad Sci U S A. 1987 Mar;84(5):1182-6. doi: 10.1073/pnas.84.5.1182.
We have previously identified by cDNA cloning 5 mRNAs that appear in resting BALB/c 3T3 cells soon after growth stimulation by serum or platelet-derived growth factor. Five additional mRNAs of this class are described in this report. The mRNAs reached peak levels between 40 and 120 min after serum addition and rapidly decayed thereafter. All 10 RNAs were superinduced in the presence of cycloheximide. Nuclear run-on experiments indicated that the increase in the mRNAs is the result of rapid transcriptional activation of their genes on stimulation by serum or platelet-derived growth factor. Superinducibility by cycloheximide is due to two effects: prolonged transcription and stabilization of mRNAs. This overall pattern of regulation is similar to that of the c-fos or c-myc protooncogenes reported previously. We hypothesize that these newly identified "immediate early" genes play a role in the proliferative response induced by growth factors.
我们之前通过cDNA克隆鉴定出了5种mRNA，它们在血清或血小板衍生生长因子刺激静止的BALB/c 3T3细胞后很快出现。本报告描述了此类另外5种mRNA。这些mRNA在添加血清后40至120分钟达到峰值水平，此后迅速衰减。在放线菌酮存在的情况下，所有10种RNA都被超诱导。细胞核连续转录实验表明，mRNA的增加是其基因在血清或血小板衍生生长因子刺激下快速转录激活的结果。放线菌酮的超诱导性归因于两种效应：转录延长和mRNA的稳定。这种整体调控模式与先前报道的c-fos或c-myc原癌基因的调控模式相似。我们推测，这些新鉴定出的“立即早期”基因在生长因子诱导的增殖反应中发挥作用。
