Department of Ultrasound Medicine, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Department of Ultrasound Medicine, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China.
Bioengineered. 2021 Dec;12(2):9832-9846. doi: 10.1080/21655979.2021.1995581.
We aim to evaluate the efficacies of combination therapy with low-frequency ultrasound-stimulated microbubbles (USMB) and radiofrequency ablation (RFA) on suppressing the proliferation of pancreatic cancer cell and treating Panc02 subcutaneous xenograft mice. The proliferation of HPDE6-C7 and Panc02 cells after the treatment of USMB and RFA alone or combination were evaluated by CCK-8 assay. Scratch test was performed to assess the cell migration capability. Panc02-bearing mice were received 14-day treatment of USMB and RFA alone or combination. Tumor size and survival rate were recorded once two days. The serum levels of immune-related factors and changes of apoptosis- and autophagy-related factors were detected by ELISA and western blotting methods. As a result, CKK-8 assays revealed significant inhibition on Panc02 cell proliferation in combination therapy with USMB and RFA relative to other groups (all < 0.05). Strong synergistic effect of USMB combined with RFA was confirmed via the calculated combination index (CI) <0.4. In addition, combination therapy of USMB and RFA significantly inhibited the migration of Panc02 cells. Moreover, combined treatment remarkably inhibited the size and width of xenograft and improved the survival in Panc02-bearing mice. Furthermore, 14-day combination therapy of USMB and RFA in Panc02-bearing mice significantly facilitated the apoptosis and autophagy of tumor cells. In summary, combination therapy of USMB and RFA showed synergistic anti-tumor efficacies on Panc02 cells attributing to the promotion on apoptosis and autophagy in Panc02 subcutaneous xenograft mice.
我们旨在评估低频超声刺激微泡（USMB）联合射频消融（RFA）治疗对抑制胰腺癌细胞增殖和治疗 Panc02 皮下移植瘤小鼠的疗效。通过 CCK-8 法评估 USMB 和 RFA 单独或联合处理后 HPDE6-C7 和 Panc02 细胞的增殖情况。划痕试验评估细胞迁移能力。将 Panc02 荷瘤小鼠接受 14 天的 USMB 和 RFA 单独或联合治疗。每两天记录一次肿瘤大小和存活率。通过 ELISA 和 Western blot 方法检测免疫相关因子的血清水平和凋亡及自噬相关因子的变化。结果显示，与其他组相比，USMB 和 RFA 联合治疗对 Panc02 细胞增殖有明显抑制作用（均<0.05）。通过计算的联合指数（CI）<0.4，证实了 USMB 与 RFA 的协同作用。此外，USMB 和 RFA 的联合治疗显著抑制了 Panc02 细胞的迁移。此外，联合治疗显著抑制了异种移植瘤的大小和宽度，并提高了 Panc02 荷瘤小鼠的存活率。此外，USMB 和 RFA 联合治疗 14 天可显著促进 Panc02 荷瘤小鼠肿瘤细胞的凋亡和自噬。综上所述，USMB 和 RFA 的联合治疗对 Panc02 细胞表现出协同的抗肿瘤疗效，这归因于对 Panc02 皮下移植瘤小鼠中凋亡和自噬的促进作用。
