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长链非编码 RNA NEAT1 的沉默通过 miR-485-5p/AIM2 轴改善子痫前期中 Treg/Th17 失衡。

Silencing of long non-coding RNA NEAT1 improves Treg/Th17 imbalance in preeclampsia via the miR-485-5p/AIM2 axis.

机构信息

Department of Obstetrics and Gynecology, Shenzhen Longhua District Central Hospital, Guangdong Medical University Affiliated Longhua District Central Hospital, Shenzhen, China.

Department of Clinical Laboratory, Shenzhen Longhua District Central Hospital, Guangdong Medical University Affiliated Longhua District Central Hospital, Shenzhen, China.

出版信息

Bioengineered. 2021 Dec;12(1):8768-8777. doi: 10.1080/21655979.2021.1982306.

DOI:10.1080/21655979.2021.1982306
PMID:34696702
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8806521/
Abstract

T-regulatory (Treg)/T-helper 17 (Th17) imbalance is associated with preeclampsia (PE). Herein, we aimed to explore the effect and mechanism of lncRNA NEAT1 on the Treg/Th17 balance. The levels of nuclear enriched abundant transcript 1 (NEAT1), miR-485-5p, and absent in melanoma 2 (AIM2) in CD4 T cells were determined using real-time quantitative polymerase chain reaction (RT-qPCR). Treg and Th17 cells were examined using flow cytometry. The relationship between miR-485-5p and NEAT1 or AIM2 was assessed using a dual-luciferase reporter assay. Pearson's correlation coefficient was used to analyze the correlation. All the data indicated that NEAT1 was upregulated in PE. The number of Treg cells decreased and was negatively related to NEAT1, whereas the number of Th17 cells increased and was positively related to NEAT1 in PE. Knockdown of NEAT1 increased the Treg cells and Treg/Th17 but decreased Th17 cells. Furthermore, NEAT1 sponges miR-485-5p to suppress the target AIM2 levels. Inhibition of miR-485-5p or upregulation of AIM2 abrogated the effect on Treg/Th17 balance induced by knockdown of NEAT1. In conclusion, silencing of NEAT1 promoted Treg/Th17 balance via the miR-485-5p/AIM2 axis in PE, suggesting that NEAT1 is a potential target for the treatment of PE.

摘要

调节性 T 细胞（Treg）/辅助性 T 细胞 17（Th17）失衡与子痫前期（PE）有关。在此，我们旨在探讨长链非编码 RNA NEAT1 对 Treg/Th17 平衡的影响和机制。采用实时定量聚合酶链反应（RT-qPCR）测定 CD4 T 细胞中核富集丰富转录物 1（NEAT1）、miR-485-5p 和黑色素瘤 2（AIM2）的水平。采用流式细胞术检测 Treg 和 Th17 细胞。采用双荧光素酶报告基因检测评估 miR-485-5p 与 NEAT1 或 AIM2 之间的关系。采用皮尔逊相关系数分析相关性。所有数据均表明，PE 中 NEAT1 上调。Treg 细胞数量减少，与 NEAT1 呈负相关，而 Th17 细胞数量增加，与 NEAT1 呈正相关。敲低 NEAT1 增加了 Treg 细胞和 Treg/Th17，但减少了 Th17 细胞。此外，NEAT1 海绵 miR-485-5p 抑制靶 AIM2 水平。抑制 miR-485-5p 或上调 AIM2 可消除敲低 NEAT1 对 Treg/Th17 平衡的影响。总之，沉默 NEAT1 通过 miR-485-5p/AIM2 轴促进 PE 中的 Treg/Th17 平衡，表明 NEAT1 是治疗 PE 的潜在靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3475/8806521/204a2536d37e/KBIE_A_1982306_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3475/8806521/0d9504f67719/KBIE_A_1982306_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3475/8806521/afc9f916176c/KBIE_A_1982306_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3475/8806521/13d792d6f48e/KBIE_A_1982306_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3475/8806521/cc3a547892ba/KBIE_A_1982306_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3475/8806521/001631f459ae/KBIE_A_1982306_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3475/8806521/204a2536d37e/KBIE_A_1982306_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3475/8806521/0d9504f67719/KBIE_A_1982306_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3475/8806521/afc9f916176c/KBIE_A_1982306_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3475/8806521/13d792d6f48e/KBIE_A_1982306_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3475/8806521/cc3a547892ba/KBIE_A_1982306_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3475/8806521/001631f459ae/KBIE_A_1982306_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3475/8806521/204a2536d37e/KBIE_A_1982306_F0006_OC.jpg

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