鱿鱼巨大轴突细胞体中钠通道的合成。
Synthesis of sodium channels in the cell bodies of squid giant axons.
作者信息
Brismar T, Gilly W F
出版信息
Proc Natl Acad Sci U S A. 1987 Mar;84(5):1459-63. doi: 10.1073/pnas.84.5.1459.
Abstract
Giant axons in squid are formed by fusion of axons from many small cell bodies in the giant fiber lobe (GFL) of the stellate ganglion. Somata of GFL cells in vivo are inexcitable and do not have measurable sodium current (INa) when studied with microelectrode or patch-electrode voltage-clamp techniques. If GFL cells are separated from the giant axons and maintained in primary culture, axon-like INa can be recorded from the somata after several days. Incorporation of Na channels into GFL cell bodies requires protein synthesis, intracellular microtubule-based transport, and the lack of a morphologically defined axon to serve as a sink for channels synthesized in culture.
摘要
鱿鱼的巨轴突是由星状神经节巨纤维叶（GFL）中许多小细胞体的轴突融合形成的。当用微电极或膜片钳电压钳技术研究时，体内GFL细胞的胞体是不可兴奋的，并且没有可测量的钠电流（INa）。如果将GFL细胞与巨轴突分离并维持在原代培养中，几天后可从胞体记录到类似轴突的INa。将钠通道整合到GFL细胞体中需要蛋白质合成、基于细胞内微管的运输，并且缺乏形态学上明确的轴突作为培养中合成通道的汇聚点。
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