A1-R 精准靶向并抑制三阴性乳腺癌 PDX 模型中的基质生成。
A1-R Exquisitely Targets and Arrests a Matrix-producing Triple-negative Breast Carcinoma in a PDOX Model.
机构信息
AntiCancer Inc, San Diego, CA, U.S.A.
Department of Surgery, University of California, San Diego, CA, U.S.A.
出版信息
In Vivo. 2021 Nov-Dec;35(6):3067-3071. doi: 10.21873/invivo.12602.
Abstract
BACKGROUND/AIM: Triple-negative matrix-producing breast carcinoma (MPBC) is rare, recalcitrant, and highly aggressive. The present study aimed to determine the efficacy of tumor-targeting leucine-arginine auxotroph Salmonella typhimurium (S. typhimurium) A1-R on a triple-negative MPBC in a patient-derived orthotopic xenograft (PDOX) model.
MATERIALS AND METHODS
The PDOX MPBC model was established in the left second mammary gland of nude mice by surgical orthotopic implantation (SOI). PDOX models were randomized into two groups when the tumor volume reached over 70 mm: a control group (n=6); and a tumor-targeting S. typhimurium A1-R group (n=7), [intravenous (i.v.) injection of S. typhimurium A1-R via the tail vein, weekly, for two weeks]. All mice were sacrificed on day 14. Tumor volume and body weight were measured once per week.
RESULTS
S. typhimurium A1-R exquisitely targeted and arrested the growth of the MPBC PDOX compared to the control group (p=0.017).
CONCLUSION
S. typhimurium A1-R has future clinical potential for triple-negative MPBC patients.
摘要
背景/目的：三阴性基质产生型乳腺癌（MPBC）罕见、难治且侵袭性高。本研究旨在评估肿瘤靶向性亮氨酸-精氨酸营养缺陷型鼠伤寒沙门氏菌（S. typhimurium）A1-R 在三阴性 MPBC 患者来源的原位异种移植（PDOX）模型中的疗效。
材料与方法
通过手术原位植入法（SOI）在裸鼠左侧第二乳腺中建立 PDOX MPBC 模型。当肿瘤体积超过 70mm 时，将 PDOX 模型随机分为两组：对照组（n=6）和肿瘤靶向性 S. typhimurium A1-R 组（n=7）[通过尾静脉静脉内（i.v.）注射 S. typhimurium A1-R，每周一次，共两周]。所有小鼠均于第 14 天处死。每周测量一次肿瘤体积和体重。
结果
与对照组相比，S. typhimurium A1-R 对 MPBC PDOX 的生长具有高度靶向性和抑制作用（p=0.017）。
结论
S. typhimurium A1-R 对三阴性 MPBC 患者具有潜在的临床应用前景。
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