Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
Department of Veterinary Pathology, Small Animal Tumor Diagnostic Center, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
J Vet Sci. 2021 Nov;22(6):e77. doi: 10.4142/jvs.2021.22.e77. Epub 2021 Sep 8.
Serum-based parameters are considered non-invasive biomarkers for cancer detection. In human studies, insulin-like growth factor-I and II (IGF-I and IGF-II) and insulin-like growth factor binding protein-3 (IGFBP-3) are useful as diagnostic or prognostic markers and potential therapeutic targets.
This study examined the diagnostic utility of circulating IGF-I, IGF-II, and IGFBP-3 levels in healthy dogs and dogs with tumors.
The serum concentrations of these biomarkers in 86 dogs with tumors were compared with those in 30 healthy dogs using an enzyme-linked immunosorbent assay (ELISA).
The ELISA results showed no difference between healthy dogs and dogs with tumors in the serum IGF-II concentrations. On the other hand, there was a significant difference in the circulating IGF-I and IGFBP-3 levels between healthy dogs and dogs with tumors. The concentrations of serum IGF-I (median [interquartile range], 103.4 [59.5-175] ng/mL) in dogs with epithelial tumors were higher than those (58.4 ng/mL [43.5-79.9]) in healthy dogs. Thus, the concentrations of serum IGFBP-3 (43.4 ng/mL [33.2-57.2]) in dogs with malignant mesenchymal tumors were lower than those (60.8 ng/mL [47.6-70.5]) in healthy dogs.
The serum IGF-I and IGFBP-3 levels can be used as diagnostic biomarkers in dogs with tumors.
基于血清的参数被认为是癌症检测的非侵入性生物标志物。在人体研究中，胰岛素样生长因子-I 和 II（IGF-I 和 IGF-II）和胰岛素样生长因子结合蛋白-3（IGFBP-3）可用作诊断或预后标志物以及潜在的治疗靶点。
本研究检查了循环 IGF-I、IGF-II 和 IGFBP-3 水平在健康犬和患有肿瘤的犬中的诊断效用。
使用酶联免疫吸附试验（ELISA）比较了 86 只患有肿瘤的犬和 30 只健康犬的这些生物标志物的血清浓度。
ELISA 结果显示，健康犬和患有肿瘤的犬之间的血清 IGF-II 浓度没有差异。另一方面，健康犬和患有肿瘤的犬之间的循环 IGF-I 和 IGFBP-3 水平存在显著差异。患有上皮肿瘤的犬的血清 IGF-I 浓度（中位数[四分位数范围]，103.4 [59.5-175]ng/mL）高于健康犬（58.4ng/mL[43.5-79.9]）。因此，患有恶性间充质肿瘤的犬的血清 IGFBP-3 浓度（43.4ng/mL[33.2-57.2]）低于健康犬（60.8ng/mL[47.6-70.5]）。
血清 IGF-I 和 IGFBP-3 水平可作为犬肿瘤的诊断生物标志物。
