Heyming Theodore, Bacon Kellie, Lara Bryan, Knudsen-Robbins Chloe, Tongol Aprille, Sanger Terence
Children's Hospital of Orange County, Orange, CA 92868, USA.
Department of Emergency Medicine, University of California, Irvine, CA 92868, USA.
Infect Dis Rep. 2021 Oct 21;13(4):910-916. doi: 10.3390/idr13040082.
The primary aim of this study was to determine the seroprevalence of SARS-CoV-2 antibodies in a population of pediatric healthcare workers (HCWs). This study was conducted 14 May-13 July 2020. Study participants included pediatric HCWs at a pediatric hospital with either direct patient contact or close proximity to patient-care areas. SARS-CoV-2 antibodies were assessed via the Wytcote Superbio SARS-CoV-2 IgM/IgG Antibody Fast Detection Kit and the Abbott Architect SARS-CoV-2 IgG assay. Participants underwent RT-PCR testing upon entry to the study and following rapid IgM+/IgG+ results; respiratory panel PCR (RP-PCR) was performed following IgM+ results. A total of 57 of 289 (19.7%) of participants demonstrated positive serology as assessed by the Wytcote rapid kit (12 on Day 1 and 45 throughout the study). However, only one of these participants demonstrated IgG+ serology via the Abbott assay. Two participants tested SARS-CoV-2+ via RT-PCR testing. One individual was adenovirus+ and enterovirus/rhinovirus+. In our study population, we observed a seroprevalence of SARS-CoV-2 antibodies of 0.35%. The lack of concordance between antibody tests suggests that the Wytcote rapid test kit may not be of use as a screening tool. However, the feasibility of the overall process indicates that a similar methodology may have potential for future epidemiologic surveillance.
本研究的主要目的是确定儿科医护人员群体中新冠病毒抗体的血清流行率。本研究于2020年5月14日至7月13日进行。研究参与者包括一家儿童医院中直接接触患者或与患者护理区域密切接触的儿科医护人员。通过Wytcote Superbio新冠病毒IgM/IgG抗体快速检测试剂盒和雅培Architect新冠病毒IgG检测法评估新冠病毒抗体。参与者在进入研究时以及快速检测IgM+/IgG+结果后接受逆转录聚合酶链反应（RT-PCR）检测；IgM+结果后进行呼吸道 panel PCR（RP-PCR）检测。在通过Wytcote快速试剂盒评估的289名参与者中，共有57名（19.7%）血清学检测呈阳性（第1天有12名，研究期间共有45名）。然而，通过雅培检测法，这些参与者中只有1名IgG+血清学检测呈阳性。两名参与者通过RT-PCR检测呈新冠病毒阳性。一名个体腺病毒阳性且肠道病毒/鼻病毒阳性。在我们的研究人群中，我们观察到新冠病毒抗体的血清流行率为0.35%。抗体检测结果缺乏一致性表明，Wytcote快速检测试剂盒可能无法用作筛查工具。然而，整个过程的可行性表明，类似的方法可能对未来的流行病学监测具有潜力。
