Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.
Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran.
Microb Drug Resist. 2022 Feb;28(2):210-216. doi: 10.1089/mdr.2021.0001. Epub 2021 Oct 25.
Fecal carriage of multidrug-resistant , particularly sequence type 131 (ST131), is becoming a global concern. This study aimed at determining the prevalence rate and molecular epidemiology of extended-spectrum -lactamase-producing (ESBL-Ec), carbapenemase-producing (CPEc), ceftazidime/avibactam (CAZ/AVI)-resistant , and ST131 isolates in healthy fecal carriers in Tehran, Iran. Among 540 samples studied, 233 (43.1%) carried ESBL-Ec, with the majority (93.9%) harboring the The carriage rate of CPEc was 2.5% ( = 14/540), and gene was the predominant carbapenemase gene. Most CPEc isolates ( = 11/14) was shown to be resistant to CAZ/AVI. Among ESBL-Ec/CPEc, 7.3% ( = 17/233) belonged to ST131 clone, which was identified by polymerase chain reaction and confirmed by multilocus sequence typing. The ST131 isolates genetically typed by pulsed-field gel electrophoresis were heterogeneous and four different plasmids were detected by plasmid typing, with the IncFIA/FIB being the major type. Our findings disclose that the presence of carbapenem-resistant ST131 isolates, which are also resistant to CAZ/AVI, contributes to the spread of resistant strains in the community. Therefore, screening and monitoring of such resistant clone in healthy people is necessary.
肠道携带多药耐药，特别是序列型 131（ST131），正在成为一个全球性的问题。本研究旨在确定伊朗德黑兰健康粪便携带者中产生超广谱β-内酰胺酶（ESBL-Ec）、产碳青霉烯酶（CPEc）、头孢他啶/阿维巴坦（CAZ/AVI）耐药和 ST131 分离株的流行率和分子流行病学。在研究的 540 个样本中，233 个（43.1%）携带 ESBL-Ec，其中大多数（93.9%）携带 基因。CPEc 的携带率为 2.5%（=14/540）， 基因是主要的碳青霉烯酶基因。大多数 CPEc 分离株（=11/14）对 CAZ/AVI 耐药。在 ESBL-Ec/CPEc 中，7.3%（=17/233）属于 ST131 克隆，通过聚合酶链反应和多位点序列分型鉴定。通过脉冲场凝胶电泳进行基因分型的 ST131 分离株具有异质性，并通过质粒分型检测到四种不同的质粒，IncFIA/FIB 是主要类型。我们的研究结果表明，耐碳青霉烯的 ST131 分离株的存在，这些分离株也对 CAZ/AVI 耐药，导致社区中耐药菌株的传播。因此，对健康人群中这种耐药克隆进行筛选和监测是必要的。
