Whole-Genome Analysis of a High-Risk Clone of ST147 Carrying Both and Genes in Peru.

The increasing prevalence and dissemination of carbapenemase-producing represent a serious concern for public health. We studied the genetic features of a multidrug-resistant isolate of high-risk clone ST147 coharboring -1 and recovered from a human clinical urine sample in 2017 in Peru. Whole-genome sequencing and conjugation assays identified and genes on two different conjugative plasmids, which belong to IncI2 and IncFIB/HI1B incompatibility groups, respectively. The presence of (in the studied isolate, located on the chromosome) and mutations in GyrA S83I and ParC S80I were detected, as expected for ST147. In addition, other β-lactamases ( and ) and PMQR ( and ) among several resistance determinants were identified. The coexistence not previously described of these genes in the same high-risk clone is a cause for serious concern that supports the need for implementation of genomic surveillance studies.