阴茎鳞状细胞癌患者循环肿瘤细胞作为治疗标志物的检测：一项初步研究。
Detection of circulating tumor cells as therapeutic markers in patients with penile squamous cell carcinoma: A preliminary study.
作者信息
Kao Chien-Chang, Xu Ting, Yang Yung-Ning, Tsai Yi-Ta, Liu Shu-Yu, Wu Sheng-Tang, Meng En, Tsao Chih-Wei, Chen Chin-Li, Sun Guang-Huan, Yu Dah-Shyong, Chang Sun-Yran, Cha Tai-Lung, Yang Ming-Hsin
机构信息
Division of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Graduate School of Medical Sciences, National Defense Medical Center, Taipei, Taiwan, ROC.
出版信息
J Chin Med Assoc. 2022 Jan 1;85(1):95-101. doi: 10.1097/JCMA.0000000000000637.
BACKGROUND
This study aimed to investigate the presence of circulating tumor cells (CTCs) in patients with penile squamous cell carcinoma (PSCC).
METHODS
CTCs were isolated from 14 patients with PSCC, 6 patients with balanoposthitis, and 6 healthy individuals. CTCs were enriched based on cell surface markers and filtered through the IsoFlux device, followed by identification according to cell morphology and immunofluorescence studies.
RESULTS
CTCs were found in all PSCC blood samples but not in balanoposthitis samples and samples from healthy individuals. Immunofluorescence studies confirmed the tumor origin. When the patients with PSCC were stratified according to metastatic inguinal lymph node status, a statistically significant difference was observed in the number of detected CTCs.
CONCLUSION
Our study showed that CTCs in PSCC may represent a valuable marker for differentiating PSCC from other tumors. Based on the correlation with some clinical parameters, CTC analysis is possibly relevant for noninvasive monitoring of disease progression and prognosis. The results also suggested a potential role of CTCs in preventing overtreatment, such as inguinal lymph node dissection.
背景
本研究旨在调查阴茎鳞状细胞癌（PSCC）患者循环肿瘤细胞（CTC）的存在情况。
方法
从14例PSCC患者、6例龟头炎患者和6名健康个体中分离CTC。基于细胞表面标志物富集CTC，并通过IsoFlux装置过滤，随后根据细胞形态和免疫荧光研究进行鉴定。
结果
在所有PSCC血液样本中均发现了CTC，但在龟头炎样本和健康个体样本中未发现。免疫荧光研究证实了肿瘤来源。当根据转移性腹股沟淋巴结状态对PSCC患者进行分层时，检测到的CTC数量存在统计学上的显著差异。
结论
我们的研究表明，PSCC中的CTC可能是区分PSCC与其他肿瘤的有价值标志物。基于与一些临床参数的相关性，CTC分析可能与疾病进展和预后的无创监测相关。结果还提示了CTC在预防过度治疗（如腹股沟淋巴结清扫）方面的潜在作用。
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