Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan,
Division of Transboundary Animal Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki 305-0856, Japan.
Avian Dis. 2021 Sep;65(3):508-515. doi: 10.1637/aviandiseases-D-21-00049.
The aim of this study was to investigate the sensitivity of three breeds of Japanese native chickens, commercial broilers, and specific-pathogen-free (SPF) white leghorns to three strains of the H5 subtype of highly pathogenic avian influenza viruses (HPAIVs). Chickens were experimentally inoculated with doses of 10, 10, and 10 50% egg infective dose of A/mandarin duck/Miyazaki/22M-765/2011 (duck-11), A/chicken/Miyazaki/7/2014 (chicken-14), and A/chicken/Kumamoto/1-2C/2016 (chicken-16). The 50% chicken lethal dose of each virus, mean death time, and viral shedding patterns were compared. The Japanese native chickens showed varied susceptibility to the three H5 HPAIV isolates. Although two of the breeds showed some degree of resistance to duck-11 and chicken-14, all three were more sensitive to chicken-16 than commercial broiler chickens. We have shown that Japanese native chickens do not necessarily have resistance to HPAIV and that the pathogenic characteristics of HPAIVs are quite different between native and commercial chickens.
本研究旨在调查三种日本本地鸡、商业肉鸡和无特定病原体（SPF）白来航鸡对三种 H5 亚型高致病性禽流感病毒（HPAIV）株的敏感性。鸡用 10、10 和 1050%卵感染剂量的 A/mandarin duck/Miyazaki/22M-765/2011（鸭-11）、A/chicken/Miyazaki/7/2014（鸡-14）和 A/chicken/Kumamoto/1-2C/2016（鸡-16）进行了实验接种。比较了每种病毒的 50%鸡致死剂量、平均死亡时间和病毒脱落模式。日本本地鸡对三种 H5 HPAIV 分离株表现出不同的易感性。尽管两种鸡种对鸭-11 和鸡-14 有一定程度的抵抗力，但与商业肉鸡相比，所有三种鸡对鸡-16 都更为敏感。我们已经表明，日本本地鸡不一定对 HPAIV 具有抗性，并且 HPAIV 的致病特征在本地鸡和商业鸡之间有很大的不同。
