孕期通过硅树脂手环评估化学暴露和内源性血浆代谢组学。
Chemical exposures assessed via silicone wristbands and endogenous plasma metabolomics during pregnancy.
机构信息
Department of Epidemiology, The Geisel School of Medicine at Dartmouth, Lebanon, NH, USA.
Nutrition Research Institute, Department of Nutrition, School of Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
出版信息
J Expo Sci Environ Epidemiol. 2022 Mar;32(2):259-267. doi: 10.1038/s41370-021-00394-6. Epub 2021 Oct 26.
BACKGROUND
Metabolomics is a promising method to investigate physiological effects of chemical exposures during pregnancy, with the potential to clarify toxicological mechanisms, suggest sensitive endpoints, and identify novel biomarkers of exposures.
OBJECTIVE
Investigate the influence of chemical exposures on the maternal plasma metabolome during pregnancy.
METHODS
Data were obtained from participants (n = 177) in the New Hampshire Birth Cohort Study, a prospective pregnancy cohort. Chemical exposures were assessed via silicone wristbands worn for one week at ~13 gestational weeks. Metabolomic features were assessed in plasma samples obtained at ~24-28 gestational weeks via the Biocrates AbsoluteIDQ® p180 kit and nuclear magnetic resonance (NMR) spectroscopy. Associations between chemical exposures and plasma metabolomics were investigated using multivariate modeling.
RESULTS
Chemical exposures predicted 11 (of 226) and 23 (of 125) metabolomic features in Biocrates and NMR, respectively. The joint chemical exposures did not significantly predict pathway enrichment, though some individual chemicals were associated with certain amino acids and related metabolic pathways. For example, N,N-diethyl-m-toluamide was associated with the amino acids glycine, L-glutamic acid, L-asparagine, and L-aspartic acid and enrichment of the ammonia recycling pathway.
SIGNIFICANCE
This study contributes evidence to the potential effects of chemical exposures during pregnancy upon the endogenous maternal plasma metabolome.
背景
代谢组学是一种有前途的方法，可以研究怀孕期间化学暴露对生理的影响，具有阐明毒理学机制、提示敏感终点和识别暴露新生物标志物的潜力。
目的
研究化学暴露对妊娠期间母体血浆代谢组的影响。
方法
数据来自新罕布什尔州出生队列研究（New Hampshire Birth Cohort Study）的参与者（n=177），这是一项前瞻性妊娠队列研究。通过在大约 13 孕周时佩戴一周的硅树脂腕带来评估化学暴露。通过 Biocrates AbsoluteIDQ® p180 试剂盒和核磁共振（NMR）光谱法在大约 24-28 孕周的血浆样本中评估代谢组学特征。使用多变量模型研究化学暴露与血浆代谢组学之间的关联。
结果
化学暴露分别预测了 Biocrates 和 NMR 中 11（226 个中）和 23（125 个中）个代谢组学特征。联合化学暴露并没有显著预测途径富集，但一些个别化学物质与某些氨基酸和相关代谢途径有关。例如，N,N-二乙基间甲苯酰胺与氨基酸甘氨酸、L-谷氨酸、L-天冬酰胺和 L-天门冬氨酸以及氨循环途径的富集有关。
意义
本研究为怀孕期间化学暴露对母体内源性血浆代谢组的潜在影响提供了证据。
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