Wickwire M A, Craig-Schmidt M C, Weete J D, Faircloth S A
J Nutr. 1987 Feb;117(2):232-41. doi: 10.1093/jn/117.2.232.
The effects of seven levels of dietary linoleic acid (18:2), with and without trans-octadecenoic acid (18:1t), on the fatty acid composition and prostaglandin content of milk were investigated in lactating rat dams. At parturition, 14 groups of 4 rats each were fed diets containing 20% fat, with mixtures of coconut and safflower oils providing seven concentrations of 18:2 ranging from 3.6 +/- 0.5 to 44.5 + 2.3% of total fatty acids. Hydrogenated soybean oil was added such that 18:1t comprised 10.3 +/- 0.3% of total fatty acids. Milk samples were collected on day 12 postpartum for prostaglandin analyses and on day 16 for fatty acid analyses. The relationship of dietary 18:2 to milk 18:2 was linear (r = 0.98; P = 0.0001), with milk 18:2 ranging from 2.6 +/- 0.1% of total fatty acids at the lowest level of dietary 18:2 to 27.9 +/- 1.6% at the highest. Milk 18:1t was highly correlated (r = 0.99; P = 0.0001) with the amount of dietary 18:1t. In rats fed hydrogenated fat, milk 18:1t averaged 7.0 +/- 0.2% of total fatty acids. Transfer from diet to milk was 46.4 +/- 4.2% for 18:2 and 37.5 +/- 1.7% for 18:1t. Mean concentrations of prostaglandin F2 alpha and E in rat milk were 269 +/- 16 pg/mL and 477 +/- 24 pg/mL respectively. There were no significant differences in the concentrations of either prostaglandin relative to 18:1t consumption at any level of 18:2.
在泌乳期大鼠母鼠中，研究了七种膳食亚油酸（18:2）水平（添加或不添加反式十八碳烯酸（18:1t））对牛奶脂肪酸组成和前列腺素含量的影响。分娩时，将14组大鼠（每组4只）喂以含20%脂肪的日粮，用椰子油和红花油的混合物提供七种浓度的18:2，其占总脂肪酸的比例范围为3.6±0.5%至44.5 + 2.3%。添加氢化大豆油，使18:1t占总脂肪酸的10.3±0.3%。产后第12天采集牛奶样本进行前列腺素分析，第16天采集样本进行脂肪酸分析。膳食18:2与牛奶18:2之间的关系呈线性（r = 0.98；P = 0.0001），牛奶18:2占总脂肪酸的比例范围从膳食18:2最低水平时的2.6±0.1%到最高水平时的27.9±1.6%。牛奶18:1t与膳食18:1t的量高度相关（r = 0.99；P = 0.0001）。在喂食氢化脂肪的大鼠中，牛奶18:1t平均占总脂肪酸的7.0±0.2%。18:2从日粮到牛奶的转化率为46.4±4.2%，18:1t为37.5±1.7%。大鼠牛奶中前列腺素F2α和E的平均浓度分别为269±16 pg/mL和477±24 pg/mL。在任何18:2水平下，两种前列腺素的浓度相对于18:1t的摄入量均无显著差异。
