在十二烷酰高丝氨酸内酯存在下铜绿假单胞菌生物膜形成动力学及弹性蛋白酶活性的研究
Study of the Dynamics of Biofilm Formation and Elastase Activity of Pseudomonas aeruginosa in the Presence of Dodecanoyl-Homoserine Lactone.
作者信息
Cherepushkina V S, Mironova T E, Afonyushkin V N, Koptev V Yu, Nefedova E V, Donchenko N A, Dimova A S
机构信息
Siberian Federal Scientific Centre of Agro-BioTechnologies, Russian Academy of Sciences, Krasnoobsk, Russia.
Novosibirsk State Agrarian University, Novosibirsk, Russia.
出版信息
Bull Exp Biol Med. 2021 Oct;171(6):741-744. doi: 10.1007/s10517-021-05307-z. Epub 2021 Oct 27.
We studied the effect of early accumulation of N-3-oxo-dodecanoyl-homoserine lactone on the suppression of Pseudomonas aeruginosa reproduction, biofilm formation, and elastase activity. N-3-oxo-dodecanoyl-homoserine lactone in various concentrations was added to the P. aeruginosa culture, and changes in the concentration of bacteria and the formation of biofilms were studied in dynamics. N-3-oxo-dodecanoyl-homoserine lactone in a concentration of 25 μM, decelerated proliferation of bacterial cells during the first 6 h of culturing (p<0.05) and stimulated biofilm formation after 18 h of culturing. Elastase activity of P. aeruginosa increased significantly after addition of N-3-oxo-dodecanoyl-homoserine lactone in a concentration of 0.75 μM.
我们研究了N-3-氧代十二烷酰高丝氨酸内酯早期积累对铜绿假单胞菌繁殖、生物膜形成及弹性蛋白酶活性的抑制作用。将不同浓度的N-3-氧代十二烷酰高丝氨酸内酯添加到铜绿假单胞菌培养物中，动态研究细菌浓度变化及生物膜形成情况。浓度为25μM的N-3-氧代十二烷酰高丝氨酸内酯在培养的前6小时减缓了细菌细胞的增殖（p<0.05），并在培养18小时后刺激了生物膜形成。添加浓度为0.75μM的N-3-氧代十二烷酰高丝氨酸内酯后，铜绿假单胞菌的弹性蛋白酶活性显著增加。
Zotero 插件