褐藻胞质分裂和胞间连丝形成的超微结构观察。

Ultrastructural Observation of Cytokinesis and Plasmodesmata Formation in Brown Algae.

机构信息

Muroran Marine Station, Field Science Center for Northern Biosphere, Hokkaido University, Muroran, Japan.

Graduate School of Environmental Science, Hokkaido University, Sapporo, Japan.

出版信息

Methods Mol Biol. 2022;2382:253-264. doi: 10.1007/978-1-0716-1744-1_16.

DOI:10.1007/978-1-0716-1744-1_16

PMID:34705245

Abstract

Similar to land plant cells, brown algal cells possess plasmodesmata with minute cytoplasmic tunnels, which enable the direct connection between adjacent cells. Plasmodesmata are distributed depending on the association of their formation with cytokinesis. Primary plasmodesmata are formed during cytokinesis, while secondary plasmodesmata appear on the cell wall septum following cytokinesis. Typically, the plasmodesmata of brown algae are cylindrical without the penetration of desmotubules from the endoplasmic reticulum, and there are no morphological differences between primary and secondary plasmodesmata. This present chapter describes the observation of cytokinesis and primary plasmodesmata formation in brown algae using electron microscopy as well as the examination of polysaccharide distribution using antibodies and enzyme-gold probes.

摘要

与陆地植物细胞类似，褐藻细胞具有带有微小细胞质隧道的胞间连丝，使相邻细胞能够直接连接。胞间连丝的分布取决于其与有丝分裂的关联。初级胞间连丝在有丝分裂过程中形成，而次级胞间连丝则在有丝分裂后出现在细胞壁隔膜上。通常，褐藻的胞间连丝是圆柱形的，没有内质网的胞质小管从中穿过，初级和次级胞间连丝之间没有形态差异。本章描述了使用电子显微镜观察褐藻的有丝分裂和初级胞间连丝形成的过程，以及使用抗体和酶金探针检查多糖分布的过程。