Piacibello W, Aglietta M, Stacchini A, Spinelli P, Salvetti L, Kerim S, Malavasi F, Infelise V, Resegotti L, Gavosto F
Leuk Res. 1987;11(3):285-90. doi: 10.1016/0145-2126(87)90053-1.
It has been suggested that the expression of some HLA class II antigens, derived from three loci (DR, DP, DQ) is important in the regulation of both the immune response and the response of haemopoietic progenitors to regulation factors, such as acidic isoferritins (AIF), as well as in the interaction between T lymphocytes and erythroid progenitors (BFU-E). Changes in the expression of class II antigens have been reported on the surface of granulo-monocyte progenitors in chronic myeloid leukemia (CML) and correlated to the abnormal proliferation of such cells. In this study, monoclonal antibodies against DR and DQ monomorphic determinants were used to investigate the expression of these antigens on the surface of normal and CML bone marrow and peripheral blood BFU-E by means of complement mediated cytotoxicity. It was found that most normal and leukemic BFU-E express DR antigens. Antigens density tends to be greater on marrow as opposed to peripheral precursors. In addition, leukemic BFU-E are more sensitive to cytolytic treatment than their normal counterparts. Normal BFU-E do not express detectable amounts of DQ antigens, whereas these are present on a proportion of leukemic BFU-E.
有人提出，源自三个基因座（DR、DP、DQ）的某些HLA II类抗原的表达，在免疫应答的调节以及造血祖细胞对调节因子（如酸性异铁蛋白（AIF））的应答中很重要，在T淋巴细胞与红系祖细胞（BFU-E）之间的相互作用中也很重要。据报道，慢性粒细胞白血病（CML）中粒-单核祖细胞表面的II类抗原表达发生了变化，且与这类细胞的异常增殖相关。在本研究中，针对DR和DQ单态决定簇的单克隆抗体被用于通过补体介导的细胞毒性作用来研究这些抗原在正常和CML骨髓及外周血BFU-E表面的表达。研究发现，大多数正常和白血病BFU-E表达DR抗原。与外周前体细胞相比，骨髓中的抗原密度往往更高。此外，白血病BFU-E比正常BFU-E对溶细胞处理更敏感。正常BFU-E不表达可检测量的DQ抗原，而一部分白血病BFU-E则表达DQ抗原。
