Department of Vegetable Science, Punjab Agricultural University, Ludhiana 141 004, India
J Genet. 2021;100.
Interspecific hybridization with 'Satputia' (bisexual and cluster bearing) can be highly useful for the introgression of cluster bearing, high yield and gynoecism in sponge gourd (monoecious and solitary bearing). However, the occurrence of self-pollination in closed flowers and anthesis of two species at different time intervals creates hindrance in interspecific hybridization. The present investigation highlighted that the reciprocal interspecific cross (Satputia × sponge gourd) is more successful for development of F hybrid and its further utilization in development of segregating generations. Pre-anthesis emasculation (28 h before anthesis) of Satputia buds in the evening and pollination with sponge gourd (PSG-9) in the morning on the day of anthesis resulted in high fruit set. Interspecific hybrids were monoecious and morphologically intermediate for most of the vegetative, flower and fruit traits. The seed of hybrid vines was vigorous than both the parents with respect to size and weight. Ample pollen production, pollen viability and high fruit set on selfing confirmed the fertility status of vines. Although pollen size was less than both the parents, but the pollen density improved in F vines. Fertile hybrids could be easily used to generate F and BCP and TCH segregating generations. In F generation, gynoecious, adroecious, andromonoecoius, monoecious and cluster bearing vines of variable length and fruit size were observed. In back cross and triple cross generations, most of the vines were monoecious except a few adroecious and gynoecious with improved fruit size, vine growth and bearing capacity. Backcross and triple cross with sponge gourd displayed a shift towards this species.
将“Satputia”(两性花和簇生)与其他物种进行杂交非常有助于南瓜(雌雄同株和单生)的簇生性、高产和雌花形成性状的导入。然而,闭花自花授粉的发生以及两个物种在不同时间间隔开花,给种间杂交带来了障碍。本研究强调,Satputia×南瓜的正反交杂种更有利于 F1 杂种的发育,并且进一步用于分离世代的开发。傍晚对 Satputia 花蕾进行开花前去雄(开花前 28 小时),然后在开花当天早上用南瓜(PSG-9)授粉,可获得较高的结实率。种间杂种为雌雄同株,在大多数营养器官、花和果实性状方面表现为中间型。杂种藤本的种子在大小和重量方面均比双亲更为旺盛。充足的花粉产量、花粉活力和自交的高结实率证实了藤本植物的可育性。尽管花粉大小小于双亲,但 F 藤本植物的花粉密度有所提高。可育杂种可轻松用于产生 F、BCP 和 TCH 分离世代。在 F1 代中,观察到雌性、雄性、雌雄同体、雌雄同株和簇生的藤本植物,长度和果实大小不一。在回交和三交世代中,大多数藤本植物为雌雄同株,除了少数雄性和雌性植物,具有改良的果实大小、藤本生长和承载能力。与南瓜的回交和三交表现出向该物种的转变。