关于整合蛋白向膜内的转移。
On the transfer of integral proteins into membranes.
作者信息
Singer S J, Maher P A, Yaffe M P
出版信息
Proc Natl Acad Sci U S A. 1987 Apr;84(7):1960-4. doi: 10.1073/pnas.84.7.1960.
Abstract
We have earlier proposed a molecular mechanism for the translocation of hydrophilic proteins across membranes that accounts for the experimental facts and meets the restrictions that we stipulate for such a mechanism. In particular, the restrictions are that translocation occurs by successive segments of the polypeptide chain and that the ionic groups of the polypeptide remain in contact with water throughout the translocation process. The evidence indicates that the transfer of integral proteins into membranes very likely uses the same molecular machinery as does the translocation of hydrophilic proteins across membranes. Here we show how the mechanism we have proposed for translocation can also be utilized in the intercalation of known types of integral proteins, accounting for their specific topologies in the membrane.
摘要
我们之前提出了一种亲水性蛋白质跨膜转运的分子机制，该机制解释了实验事实，并符合我们为这种机制规定的限制条件。具体而言，这些限制条件是转运通过多肽链的连续片段发生，并且多肽的离子基团在整个转运过程中始终与水接触。证据表明，整合蛋白插入膜中很可能使用与亲水性蛋白跨膜转运相同的分子机制。在这里，我们展示了我们提出的转运机制如何也可用于已知类型整合蛋白的嵌入，解释它们在膜中的特定拓扑结构。
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本文引用的文献
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