Abrantes Pedro M D S, Fisher Randall, Bouic Patrick J D, McArthur Carole P, Fielding Burtram C, Africa Charlene W J
Maternal Endogenous Infections Studies (MEnIS) Research Laboratories, Department of Medical Biosciences, University of the Western Cape, Private Bag X17, Bellville 7535, South Africa.
Separated Sector Cyclotron Laboratory, iThemba LABS, Radiation Biophysics Division, National Research Foundation, Cape Town, South Africa.
AIMS Microbiol. 2021 Sep 10;7(3):320-335. doi: 10.3934/microbiol.2021020. eCollection 2021.
The objective of this study was to elucidate the proteomic mechanisms of drug resistance in HIV-infected African patients. Cell membrane fractions from forty oral isolates isolated from African HIV-positive patients were analysed using HPLC-MS with the aim of identifying proteins associated with their pathogenicity and drug resistance. Heat shock proteins that mediate the fungicidal activity of salivary peptides were found in all tested fractions, with pH-responsive proteins associated with increased pathogenicity only being present in the three most commonly isolated species. ABC multidrug transporter efflux pumps and estrogen binding proteins were only found in fractions, while ergosterol biosynthesis proteins were identified in four species. The combination of various adherence, invasion, upregulation and efflux pump mechanisms appear to be instrumental for the host colonization and drug resistance emergence in HIV-infected individuals.
本研究的目的是阐明感染HIV的非洲患者耐药性的蛋白质组学机制。对从非洲HIV阳性患者中分离出的40株口腔分离株的细胞膜组分进行了HPLC-MS分析，旨在鉴定与其致病性和耐药性相关的蛋白质。在所有测试组分中均发现了介导唾液肽杀菌活性的热休克蛋白，仅在三种最常见的分离物种中存在与致病性增加相关的pH响应蛋白。ABC多药转运蛋白外排泵和雌激素结合蛋白仅在部分组分中发现，而麦角固醇生物合成蛋白在四种物种中被鉴定。各种黏附、侵袭、上调和外排泵机制的组合似乎对HIV感染者的宿主定植和耐药性出现起作用。
