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基于16S rRNA高通量测序的北运河水体与沉积物微生物群落组成对比分析

[Contrasting Analysis of Microbial Community Composition in the Water and Sediments of the North Canal Based on 16S rRNA High-Throughput Sequencing].

作者信息

Peng Ke, Dong Zhi, Di Yan-Ming, Guo Xiao-Yu

机构信息

College of Resource Environment and Tourism, Capital Normal University, Beijing 100048, China.

Sohool of Life Sciences, Peking University, Beijing 100871, China.

出版信息

Huan Jing Ke Xue. 2021 Nov 8;42(11):5424-5432. doi: 10.13227/j.hjkx.202104122.

DOI:10.13227/j.hjkx.202104122
PMID:34708981
Abstract

As the bridge of pollutant exchange between sediments and aquatic ecosystems, microorganisms play an important role in material circulation. However, there are few comparative studies of microorganisms in water and sediment of urban rivers with unconventional water supply, sluice dam, and lining closure. The highly artificial area of Beijing-Tianjin-Hebei section of the North Canal was chosen for this study. We analyze the differences of microbial community composition in water and sediment using high-throughput sequencing. The results show that the microbial communities in the sediments of the North Canal have higher -diversity than those in the water. With regards to -diversity, the similarity of microbial communities in the water is higher than that in the sediment. There is no significant difference in the abundance of Proteobacteria between water and sediments. The abundance of -Proteobacteria, Actinobacteria, Cyanobacteria, and Verrucomicrobia was higher in water than that of sediment, while the abundance of -Proteobacteria, -Proteobacteria, Chloroflexi, Firmicutes, and Acidobacteria was higher in sediments than that of water. Aerobic or facultative anaerobes dominated the microbial aquatic system, while anaerobes dominated the sediments. The risk of bacteria releasing pathogens from the sediment into the water habitat is high. The research results provide a scientific basis for revealing the mechanism of microbial community change under river pollution risk in highly artificial reclaimed water.

摘要

作为沉积物与水生生态系统之间污染物交换的桥梁,微生物在物质循环中发挥着重要作用。然而,对于非常规供水、水闸坝和衬砌封闭的城市河流的水体和沉积物中的微生物,鲜有比较研究。本研究选取了北运河京津冀段人工化程度高的区域。我们利用高通量测序分析水体和沉积物中微生物群落组成的差异。结果表明,北运河沉积物中的微生物群落比水体中的具有更高的多样性。就多样性而言,水体中微生物群落的相似性高于沉积物中的。水体和沉积物中变形菌门的丰度没有显著差异。β-变形菌纲、放线菌门、蓝细菌门和疣微菌门在水体中的丰度高于沉积物,而α-变形菌纲、δ-变形菌纲、绿弯菌门、厚壁菌门和酸杆菌门在沉积物中的丰度高于水体。需氧或兼性厌氧菌在水生微生物系统中占主导,而厌氧菌在沉积物中占主导。沉积物中的细菌向水生生境释放病原体的风险很高。研究结果为揭示高度人工化再生水条件下河流污染风险下微生物群落变化机制提供了科学依据。

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