Gaurav Shubham, Deka Pankaj, Das Sangeeta, Deka Pubaleem, Hazarika Ritam, Kakati Parikshit, Kumar Aman, Kumar Sachin
Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, India.
Department of Veterinary Microbiology, College of Veterinary Science, Assam Agricultural University, Guwahati, India.
Avian Pathol. 2022 Feb;51(1):45-50. doi: 10.1080/03079457.2021.1999388. Epub 2021 Dec 9.
Newcastle disease virus (NDV) affects commercial poultry as well as other avian species in the wild and in captivity. Although the diversity of NDV in domestic chickens has been well understood, little light has been shed on NDV outbreaks in other avian species. We provide an annotated sequence of NDV/Owl/Guwahati/01/20, a virulent strain of NDV isolated from barn owls in captivity from Guwahati in Northeast India. The complete genome is 15,192 base pairs long with a fusion protein (F) cleavage site Q↓F. The isolate showed 97.67% identity with its closest match, another highly virulent strain from Indonesia isolated from vaccinated commercial chickens; however, they differ in the F cleavage site. The NDV isolate from the owl shares 83.02% and 81.88% identity with the vaccine strains R2B and LaSota, respectively. Phylogenetic analysis with F gene as well as whole-genome nucleotide sequence reveals that the NDV isolate from owl belongs to genotype VII, subgenotype VII.2, and differs significantly from all other isolates of NDV from India.
新城疫病毒（NDV）可感染商业家禽以及野生和圈养环境中的其他禽类。尽管家鸡中新城疫病毒的多样性已得到充分了解，但对于其他禽类中的新城疫病毒爆发情况却知之甚少。我们提供了NDV/Owl/Guwahati/01/20的注释序列，这是一种从印度东北部古瓦哈蒂圈养的仓鸮中分离出的新城疫病毒强毒株。其完整基因组长度为15,192个碱基对，融合蛋白（F）裂解位点为Q↓F。该分离株与其最相似的毒株（另一种从印度尼西亚接种疫苗的商业鸡中分离出的高致病性毒株）具有97.67%的同一性；然而，它们在F裂解位点上存在差异。从猫头鹰分离出的新城疫病毒分离株与疫苗株R2B和LaSota的同一性分别为83.02%和81.88%。以F基因以及全基因组核苷酸序列进行的系统发育分析表明，从猫头鹰分离出的新城疫病毒分离株属于VII型，VII.2亚型，并且与来自印度的所有其他新城疫病毒分离株有显著差异。
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