Institute of Stomatology, Binzhou Medical University, No. 346 Guanhai Street, Yantai, 264003, Shandong, China.
Department of Pediatrics and Preventive Dentistry, Hospital Affiliated to Binzhou Medical University, Binzhou, 256600, Shandong, China.
J Mol Histol. 2022 Feb;53(1):111-118. doi: 10.1007/s10735-021-10026-w. Epub 2021 Oct 28.
At maturation stage of enamel development, a specialized basal lamina (sBL) was built between ameloblasts and enamel. After the teeth eruption, the ameloblasts transform into the inner cell layer of junctional epithelium. The inner cell layer forms the internal basal lamina of junctional epithelium. However, the composition of the sBL and internal basal lamina was not clarified. The objective of our study was to make a description of the localization of amelotin (AMTN), laminin γ2 (LAMC2) and Odontogenesis-associated phosphoprotein (ODAPH) on the sBL and internal basal lamina. In immunohistochemical study, AMTN, LAMC2 and ODAPH were detected on the sBL at maturation stage. AMTN was also detected in ameloblasts at maturation stage. The expression of AMTN decreased from early-to-late maturation stage. In contrast, the expression of LAMC2 and ODAPH was stable. Immunofluorescence double-staining showed the localization of AMTN was close to enamel surface. However, the localization of ODAPH was close to ameloblasts. LAMC2 and ODAPH were observed on internal basal lamina of junctional epithelium. In contrast, no expression of AMTN was detected on internal basal lamina of junctional epithelium. Our results suggested that ODAPH might participate in enamel maturation and periodontal health, which might provide a better understanding of enamel defects and periodontal disease in clinic.
在釉质发育的成熟阶段，在成釉细胞和釉质之间形成了一种特殊的基底层（sBL）。牙齿萌出后，成釉细胞转化为结合上皮的内细胞层。内细胞层形成结合上皮的内基底层。然而，sBL 和内基底层的组成尚不清楚。本研究的目的是描述釉质蛋白（AMTN）、层粘连蛋白 γ2（LAMC2）和牙发生相关磷蛋白（ODAPH）在 sBL 和内基底层的定位。在免疫组织化学研究中，在成熟阶段的 sBL 上检测到 AMTN、LAMC2 和 ODAPH。在成熟阶段的成釉细胞中也检测到 AMTN。AMTN 的表达从早期到晚期成熟阶段逐渐减少。相比之下，LAMC2 和 ODAPH 的表达是稳定的。免疫荧光双重染色显示 AMTN 的定位接近釉质表面。然而，ODAPH 的定位接近成釉细胞。LAMC2 和 ODAPH 观察到在结合上皮的内基底层。相比之下，在结合上皮的内基底层未检测到 AMTN 的表达。我们的结果表明，ODAPH 可能参与釉质成熟和牙周健康，这可能为临床中釉质缺陷和牙周病提供更好的理解。
