Conde Jonas Nascimento, Mladinich Megan, Schutt William, Mackow Erich R
Department of Microbiology and Immunology, Renaissance School of Medicine, Stony Brook University, Stony Brook, NY, USA.
Department of Microbiology and Immunology, Stony Brook University, Stony Brook, NY, USA.
Methods Mol Biol. 2022;2409:197-205. doi: 10.1007/978-1-0716-1879-0_13.
A growing body of evidence demonstrates that endothelial cells (ECs) play a prominent role in immune-enhanced pathology seen in dengue virus (DENV) infection that might contribute to vascular permeability and hemorrhagic manifestations in severe dengue cases. However, it remains a question of whether DENV infection of ECs directly causes permeability or if extra-endothelial factors such as immune cell activation or antibody-dependent enhancement (ADE) are required. In this chapter, we detail the measurement of the transendothelial electrical resistance (TEER), a quantitative technique to measure the integrity of tight junction dynamics in cell culture models of endothelial monolayers and show that DENV infection of ECs does not cause endothelial permeability in vitro.
越来越多的证据表明，内皮细胞（ECs）在登革病毒（DENV）感染中出现的免疫增强病理过程中发挥着重要作用，这可能导致重症登革热病例中的血管通透性增加和出血表现。然而，ECs感染DENV是直接导致通透性增加，还是需要诸如免疫细胞激活或抗体依赖增强（ADE）等内皮外因素，仍是一个问题。在本章中，我们详细介绍了跨内皮电阻（TEER）的测量方法，这是一种用于测量内皮单层细胞培养模型中紧密连接动力学完整性的定量技术，并表明ECs感染DENV在体外不会导致内皮通透性增加。
