Comparison between the results of a recently-developed biological weighting function (V79-RBEBWF) and theclonogenic survival RBEof other repair-competent asynchronized normoxic mammalian cell lines and ions not used for the development of the model.

728 simulated microdosimetric lineal energy spectra (26 different ions betweenH andU, 28 energy points from 1 to 1000 MeV/n) were used in combination with a recently-developed biological weighting function (Parisi20201361-6560) and 571 publishedclonogenic survival curves in order to: (1) assess prediction intervals for theresults by deriving an empirical indication of the experimental uncertainty from the dispersion in thehamster lung fibroblast (V79) data used for the development of the biophysical model; (2) explore the possibility of modeling the relative biological effectiveness (RBE) of the 10% clonogenic survival of asynchronized normoxic repair-competent mammalian cell lines other than the one used for the development of the model (V79); (3) investigate the predictive power of the model through a comparison betweenresults anddata for 10 ions not used for the development of the model. At first, different strategies for the assessment of theprediction intervals were compared. The possible sources of uncertainty responsible for the dispersion in thedata were also shortly reviewed. Secondly, also because of the relevant scatter in thedata, no statistically-relevant differences were found between the RBEof the investigated different asynchronized normoxic repair-competent mammalian cell lines. The only exception (Chinese Hamster peritoneal fibroblasts, B14FAF28), is likely due to the limited dataset (allion data were extracted from a single publication), systematic differences in the linear energy transfer calculations for the employed very-heavy ions, and the use of reference photon survival curves extracted from a different publication. Finally, thepredictions for the 10 ions not used for the model development were in good agreement with the correspondingdata.