Trinchieri G, Rosen M, Perussia B
Cancer Res. 1987 May 1;47(9):2236-42.
We analyzed the combined effect of tumor necrosis factor and 1 alpha,25-dihydroxyvitamin D3 on the differentiation of human myeloid cell lines HL-60, ML3, and U937. The two compounds synergize in inducing the morphological, phenotypic, enzymatic, and functional characteristics of cells of the monocytic lineage. Immune gamma-interferon synergizes with each compound to induce differentiation. However, recombinant tumor necrosis factor is much more effective than recombinant gamma-interferon in potentiating the effect of 1 alpha,25-dihydroxyvitamin D3 and, alone, is also more effective than recombinant gamma-interferon in inducing expression of the high-affinity Fc receptor on ML3 cells. The possible physiological or pathological relevance of the synergistic effect of tumor necrosis factor and 1 alpha,25-dihydroxyvitamin D3 on monocytic differentiation is discussed.
我们分析了肿瘤坏死因子与1α,25 - 二羟基维生素D3对人髓样细胞系HL - 60、ML3和U937分化的联合作用。这两种化合物在诱导单核细胞系细胞的形态、表型、酶学和功能特性方面具有协同作用。免疫γ干扰素与每种化合物协同作用以诱导分化。然而，重组肿瘤坏死因子在增强1α,25 - 二羟基维生素D3的作用方面比重组γ干扰素更有效，并且单独使用时，在诱导ML3细胞上高亲和力Fc受体的表达方面也比重组γ干扰素更有效。本文讨论了肿瘤坏死因子与1α,25 - 二羟基维生素D3对单核细胞分化的协同作用可能具有的生理或病理相关性。
