Lin Qiu Jun, Chu Jin, Kumar Vikranth, Yuan De Peng, Li Zhi Min, Mei Qiong, Xuan Yuan Hu
College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
Institute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang, China.
Front Genome Ed. 2021 Feb 25;3:632136. doi: 10.3389/fgeed.2021.632136. eCollection 2021.
Rice () production is damaged to a great extent by sheath blight disease (ShB). However, the defense mechanism in rice against this disease is largely unknown. Previous transcriptome analysis identified a significantly induced eukaryotic () after the inoculation of . Five genes encoding PP2A exist in rice genome, and these five genes are ubiquitously expressed in different tissues and stages. Inoculation of showed that the genome edited mutants using the CRISPR/Cas9 were more susceptible to ShB than the wild-type control, but other gene mutants exhibited similar response to ShB compared to wild-type plants. In parallel, expression level was higher in the activation tagging line, and overexpression inhibited plant height and promoted the resistance to ShB. PP2A-1-GFP was localized in the cytoplasm and nucleus. In addition, -dependent induction kinetics of pathogen-related genes and was lower in mutants but higher in activation line compared to those in the wild-type. In conclusion, our analysis shows that PP2A-1 is a member of protein phosphatase, which regulates rice resistance to ShB. This result broadens the understanding of the defense mechanism against ShB and provides a potential target for rice breeding for disease resistance.
水稻（）生产受到纹枯病（ShB）的极大损害。然而，水稻针对这种疾病的防御机制在很大程度上尚不清楚。先前的转录组分析在接种后鉴定出一个显著诱导的真核生物（）。水稻基因组中存在五个编码PP2A的基因，这五个基因在不同组织和阶段普遍表达。接种表明，使用CRISPR/Cas9进行基因组编辑的突变体比野生型对照更易感染纹枯病，但其他基因突变体与野生型植物相比对纹枯病表现出相似的反应。同时，激活标签系中的表达水平更高，过表达抑制株高并增强对纹枯病的抗性。PP2A-1-GFP定位于细胞质和细胞核。此外，与野生型相比，病原体相关基因和的依赖诱导动力学在突变体中较低，但在激活系中较高。总之，我们的分析表明PP2A-1是蛋白磷酸酶的一个成员，它调节水稻对纹枯病的抗性。这一结果拓宽了对纹枯病防御机制的理解，并为水稻抗病育种提供了一个潜在靶点。
