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内源性 GapC1 和 GS 启动子的特性分析及其在三角褐指藻中重组蛋白表达。

Characterization of endogenous promoters of GapC1 and GS for recombinant protein expression in Phaeodactylum tricornutum.

机构信息

Natural Product Informatics Research Center, KIST Gangneung Institute of Natural Products, Gangneung, Republic of Korea.

Division of Bio-Medical Science and Technology, KIST School, Korea University of Science and Technology, Seoul, Republic of Korea.

出版信息

Microbiologyopen. 2021 Oct;10(5):e1239. doi: 10.1002/mbo3.1239.

DOI:10.1002/mbo3.1239
PMID:34713604
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8545674/
Abstract

Although diatoms have been utilized as a cellular factory to produce biopharmaceuticals, recombinant proteins, and biofuels, only a few numbers of gene promoters are available. Therefore, the development of novel endogenous promoters is essential for the production of a range of bioactive substances. Here, we characterized the activities of endogenous promoters glyceraldehyde-3-phosphate dehydrogenase (GapC1) and glutamine synthetase (GS) of Phaeodactylum tricornutum using green fluorescent protein (GFP) under different culture conditions. Compared with the widely used fucoxanthin chlorophyll-binding protein A (fcpA) promoter, the GS promoter constitutively drove the expression of GFP throughout all growth phases of P. tricornutum, regardless of culture conditions. Additionally, the GFP level driven by the GapC1 promoter was the highest at the log phase, similar to the fcpA promoter, and increased light and nitrogen-starvation conditions reduced GFP levels by inhibiting promoter activity. These results suggested that the GS promoter could be utilized as a strong endogenous promoter for the genetic engineering of P. tricornutum.

摘要

虽然硅藻已被用作生产生物制药、重组蛋白和生物燃料的细胞工厂，但可用的基因启动子数量有限。因此，开发新型内源性启动子对于生产一系列生物活性物质至关重要。在这里，我们使用绿色荧光蛋白 (GFP) 研究了角毛藻内源性甘油醛-3-磷酸脱氢酶 (GapC1) 和谷氨酰胺合成酶 (GS) 启动子在不同培养条件下的活性。与广泛使用的岩藻黄素叶绿素结合蛋白 A (fcpA) 启动子相比，GS 启动子在角毛藻的所有生长阶段都持续驱动 GFP 的表达，无论培养条件如何。此外，GapC1 启动子驱动的 GFP 水平在对数生长期最高，与 fcpA 启动子相似，增加光照和氮饥饿条件会通过抑制启动子活性降低 GFP 水平。这些结果表明，GS 启动子可作为角毛藻遗传工程的强内源性启动子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd17/8545674/4dd607cc1cf2/MBO3-10-e1239-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd17/8545674/9b05da2b2734/MBO3-10-e1239-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd17/8545674/dca23640a8f6/MBO3-10-e1239-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd17/8545674/59fc5441cef1/MBO3-10-e1239-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd17/8545674/a35f1a973c18/MBO3-10-e1239-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd17/8545674/b07573d2957a/MBO3-10-e1239-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd17/8545674/a0dd5a77ae0c/MBO3-10-e1239-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd17/8545674/d3a70240f291/MBO3-10-e1239-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd17/8545674/a47508d8bc43/MBO3-10-e1239-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd17/8545674/902cf65d391b/MBO3-10-e1239-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd17/8545674/4dd607cc1cf2/MBO3-10-e1239-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd17/8545674/9b05da2b2734/MBO3-10-e1239-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd17/8545674/dca23640a8f6/MBO3-10-e1239-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd17/8545674/59fc5441cef1/MBO3-10-e1239-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd17/8545674/a35f1a973c18/MBO3-10-e1239-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd17/8545674/b07573d2957a/MBO3-10-e1239-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd17/8545674/a0dd5a77ae0c/MBO3-10-e1239-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd17/8545674/d3a70240f291/MBO3-10-e1239-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd17/8545674/a47508d8bc43/MBO3-10-e1239-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd17/8545674/902cf65d391b/MBO3-10-e1239-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd17/8545674/4dd607cc1cf2/MBO3-10-e1239-g004.jpg

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3
Rapid induction of GFP expression by the nitrate reductase promoter in the diatom Phaeodactylum tricornutum.
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4
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Sci Rep. 2016 Apr 25;6:24951. doi: 10.1038/srep24951.
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