Beijing National Laboratory for Molecular Sciences (BNLMS), State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China.
Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Polymer Physics and Chemistry, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.
Langmuir. 2021 Nov 9;37(44):13125-13131. doi: 10.1021/acs.langmuir.1c02303. Epub 2021 Oct 29.
Enzyme-instructed self-assembly is an increasingly attractive topic owing to its broad applications in biomaterials and biomedicine. In this work, we report an approach to construct enzyme-responsive aqueous surfactant two-phase (ASTP) systems serving as enzyme substrates by using a cationic surfactant (myristoylcholine chloride) and a series of anionic surfactants. Driven by the hydrophobic interaction and electrostatic attraction, self-assemblies of cationic-anionic surfactant mixtures result in biphasic systems containing condensed lamellar structures and coexisting dilute solutions, which turn into homogeneous aqueous phases in the presence of hydrolase (cholinesterase). The enzyme-sensitive ASTP systems reported in this work highlight potential applications in the active control of biomolecular enrichment/release and visual detection of cholinesterase.
酶指导的自组装由于其在生物材料和生物医学中的广泛应用而成为一个日益引人关注的话题。在这项工作中，我们报告了一种构建酶响应的水相表面活性剂两相（ASTP）系统的方法，该系统可用作酶底物，使用阳离子表面活性剂（肉豆蔻酰胆碱氯化物）和一系列阴离子表面活性剂。受疏水相互作用和静电吸引的驱动，阳离子-阴离子表面活性剂混合物的自组装导致包含凝聚层状结构和共存稀溶液的双相系统，在存在水解酶（胆碱酯酶）时变成均相水相。本文报道的酶敏感 ASTP 系统突出了在生物分子富集/释放的主动控制和胆碱酯酶的可视化检测方面的潜在应用。
