人胚胎干细胞两个性别不一致的同胞系的无外源和无饲养层来源

Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells.

作者信息

Souralova Tereza, Holubcova Zuzana, Kyjovska Drahomira, Hampl Ales, Koutna Irena

机构信息

Masaryk University, Faculty of Medicine, Department of Histology and Embryology, Brno, Czech Republic; St. Anne's University Hospital, International Clinical Research Center, Cell and Tissue Engineering Facility, Brno, Czech Republic.

Masaryk University, Faculty of Medicine, Department of Histology and Embryology, Brno, Czech Republic; Reprofit International, Clinic of Reproductive Medicine, Brno, Czech Republic.

出版信息

Stem Cell Res. 2021 Dec;57:102574. doi: 10.1016/j.scr.2021.102574. Epub 2021 Oct 15.

DOI:10.1016/j.scr.2021.102574

PMID:34715503

Abstract

Human embryonic stem cells (hESCs) represent a virtually unlimited source of cells suitable for a variety of biomedical applications. However, a diminishing allogeneic background and undefined culture conditions are essential for developing robust and replicable protocols for differentiation experiments, disease modeling, and drug testing. Therefore, here we report the generation of the two sex-discordant sibling hESC lines, MUNIe008-A and MUNIe009-A, using the mechanical biopsy of vitrified-thawed embryos under xeno- and feeder-free conditions. The presented approach is applicable for deriving high-quality clinical-grade hESC lines for cell replacement therapies.

摘要

人类胚胎干细胞（hESCs）代表了一种几乎无限的细胞来源，适用于多种生物医学应用。然而，减少异基因背景和明确培养条件对于开发用于分化实验、疾病建模和药物测试的强大且可重复的方案至关重要。因此，在此我们报告了在无血清和无饲养层条件下，通过对玻璃化冷冻解冻胚胎进行机械活检，生成了两个性别不一致的同胞hESC系，即MUNIe008-A和MUNIe009-A。所提出的方法适用于获得用于细胞替代疗法的高质量临床级hESC系。