Department of Orthopaedics, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, 519000, Guangdong, China.
Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, 519000, Guangdong, China.
J Orthop Surg Res. 2021 Oct 30;16(1):647. doi: 10.1186/s13018-021-02794-8.
Rheumatoid arthritis (RA) is a chronic autoimmune disease, which commonly affects women. Accumulating evidence shows that differentially expressed circular RNAs (circRNAs) play crucial roles in the progress of RA. However, the roles of circRNAs in female RA remains unclear. This study explores potential role and diagnostic value of hsa_circ_0140271 from peripheral blood mononuclear cells (PBMC) in female RA.
Differential expression of circRNAs was determined by RNA-sequencing in PBMC from 4 healthy controls (HC) and 4 RA patients, and we further measured the level of hsa_circ_0140271 in a validation cohort consisting of 47 RA and 47 HC via RT-qPCR. Besides, correlation studies with clinical variables were also examined. What's more, we performed bioinformatics analysis to predict the potential role of hsa_circ_0140271.
PBMC expression of hsa_circ_0140271 of female RA was significantly higher than that of female HC, and it was positively correlated with antistreptolysin (ASO). Furthermore, the receiver operating characteristic (ROC) curve indicated that hsa_circ_0140271 could distinguish female RA from female HC and female patients with ankylosing spondylitis (AS) or osteoarthritis (OA). Besides, the combined diagnosis anti-cyclic citrullinated peptide (Anti-CCP) + hsa_circ_0140271 could improve diagnostic accuracy with an area under the curve (AUC) of 0.818 to compared with Anti-CCP. Furthermore, KEGG pathway enrichment analysis indicated hsa_circ_0140271 may act as microRNA sponge and participate in fatty acid metabolism pathways.
Hsa_circ_0140271 was likely to be used as a promising diagnostic biomarker for female RA; it may act as microRNA sponge to regulate fatty acid metabolism pathways in RA.
类风湿关节炎（RA）是一种常见于女性的慢性自身免疫性疾病。越来越多的证据表明，差异表达的环状 RNA（circRNA）在 RA 的进展中发挥着关键作用。然而，circRNA 在女性 RA 中的作用尚不清楚。本研究探讨了外周血单个核细胞（PBMC）中 hsa_circ_0140271 在女性 RA 中的潜在作用和诊断价值。
通过 RNA-seq 测定 4 名健康对照（HC）和 4 名 RA 患者 PBMC 中的 circRNA 差异表达，并用 RT-qPCR 检测验证队列中 47 名 RA 和 47 名 HC 的 hsa_circ_0140271 水平。此外，还进行了与临床变量的相关性研究。更重要的是，我们进行了生物信息学分析以预测 hsa_circ_0140271 的潜在作用。
女性 RA 的 PBMC 表达 hsa_circ_0140271 明显高于女性 HC，与抗链球菌溶血素（ASO）呈正相关。此外，受试者工作特征（ROC）曲线表明 hsa_circ_0140271 可区分女性 RA 与女性 HC 以及女性强直性脊柱炎（AS）或骨关节炎（OA）患者。此外，抗环瓜氨酸肽（Anti-CCP）+hsa_circ_0140271 的联合诊断可提高诊断准确性，曲线下面积（AUC）为 0.818，优于 Anti-CCP。此外，KEGG 通路富集分析表明 hsa_circ_0140271 可能作为 microRNA 海绵并参与脂肪酸代谢途径。
hsa_circ_0140271 可能作为女性 RA 的有前途的诊断生物标志物；它可能作为 microRNA 海绵来调节 RA 中的脂肪酸代谢途径。
