Hainan Provincial Key Laboratory of Tropical Maricultural Technologies, Hainan Academy of Ocean and Fisheries Sciences, Haikou, 571126, China.
Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, China.
Fish Shellfish Immunol. 2021 Dec;119:554-562. doi: 10.1016/j.fsi.2021.10.040. Epub 2021 Oct 28.
Diseases caused by Vibrio harveyi in shrimps have gradually become one group of the most serious threats to shrimp production, while related molecular mechanisms of infections with Vibrio harveyi are still not known well in shrimps. Here, we performed proteomic sequencing of hepatopancreas in whiteleg shrimps (Litopenaeus vannamei) infected with exogenous Vibrio harveyi, and subsequent functional annotation and calculation of differentially expressed proteins (DEPs) in this study. A total of 145 DEPs were obtained, among them 36 were up-regulated and 109 were down-regulated after the infection. Meanwhile, our results showed that after the infection of Vibrio harveyi, expression levels of a variety of C-type lectins (CTLs) were changed significantly. In-depth functional domain analysis and spatial structure prediction of these CTLs revealed that amino acid sequences and spatial structures of the C-type lectin domain (CTLD) shared by the CTL-S and IML proteins were variant, suggesting differential functions between the two CTLs. In summary, various members of the CTL family have different epidemic responses to Vibrio harveyi infection, which provides a theoretical guidance for deep-going investigations on practical immunity reactions and pathogen infections in shrimps.
哈维弧菌引起的虾病已逐渐成为对虾养殖业最严重的威胁之一，而虾类感染哈维弧菌的相关分子机制仍不清楚。本研究通过对感染外源哈维弧菌的凡纳滨对虾（Litopenaeus vannamei）的肝胰腺进行蛋白质组测序，并对差异表达蛋白（DEPs）进行后续功能注释和计算。共获得 145 个 DEPs，其中感染后上调 36 个，下调 109 个。同时，研究结果表明，哈维弧菌感染后，多种 C 型凝集素（CTLs）的表达水平发生明显变化。对这些 CTLs 进行深入的功能结构域分析和空间结构预测表明，CTL-S 和 IML 蛋白的 C 型凝集素域（CTLD）的氨基酸序列和空间结构存在差异，提示两种 CTL 具有不同的功能。总之，CTL 家族的各种成员对哈维弧菌感染的流行反应不同，为深入研究虾类的实际免疫反应和病原体感染提供了理论指导。
