利用连接介导的复合物策略和酵母遗传互补分析组蛋白变体 H2A.Z 的分子进化。

Analysis of the molecular evolution of histone variant H2A.Z using a linker-mediated complex strategy and yeast genetic complementation.

机构信息

Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, Miyagi, Japan.

Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University, Aoba-ku, Sendai, Miyagi, Japan.

出版信息

Biosci Biotechnol Biochem. 2021 Dec 22;86(1):104-108. doi: 10.1093/bbb/zbab190.

DOI:10.1093/bbb/zbab190

PMID:34718407

Abstract

The histone variant H2A.Z is deposited into chromatin by specific machinery and is required for genome functions. Using a linker-mediated complex strategy combined with yeast genetic complementation, we demonstrate evolutionary conservation of H2A.Z together with its chromatin incorporation and functions. This approach is applicable to the evolutionary analyses of proteins that form complexes with interactors.

摘要

组蛋白变体 H2A.Z 通过特定的机制被沉积到染色质中，并且对于基因组功能是必需的。我们使用连接介导的复合物策略结合酵母遗传互补，证明了 H2A.Z 与其染色质掺入和功能的进化保守性。这种方法适用于与相互作用蛋白形成复合物的蛋白质的进化分析。