O'Connor R, Bradley J G, Lawlor E, Cotter T G
Leuk Res. 1987;11(5):481-8. doi: 10.1016/0145-2126(87)90081-6.
A monoclonal antibody termed NC-1 was produced which binds to an antigen present on the human promyelocytic leukemia cell line HL-60 and peripheral blood neutrophils. The antibody bound to the majority of promyelocytes in the HL-60 culture, but not to myeloblasts. No antibody reactivity was detected to a range of other cell lines or to a limited number of normal human tissues. Peripheral blood lymphocytes, monocytes, basophils, eosinophils, erythrocytes and platelets did not react with the antibody. Bone marrow smears exhibited binding of NC-1 to myeloid cells at the promyelocyte and later stages of differentiation along the granulocyte lineage. The KG-1 myeloblastoid and U937 myelomonocytic lines could be induced to express the antigen, when exposed to neuraminidase which removes terminal sialic acid from carbohydrate residues. Similarly myeloblasts in bone marrow samples bound NC-1 when they were treated with neuraminidase. HL-60 cells induced to differentiate to granulocytes by treatment with retinoic acid continued to express the antigen. A similar result was observed when HL-60 cells were induced to differentiate to monocytes, even though blood monocytes failed to bind the antibody. These data indicate that the antibody NC-1 reacts with an antigen expressed on myeloid cells beyond the promyelocyte stage of differentiation.
产生了一种名为NC-1的单克隆抗体，它能与人早幼粒细胞白血病细胞系HL-60及外周血中性粒细胞上存在的一种抗原结合。该抗体与HL-60培养物中的大多数早幼粒细胞结合，但不与原始粒细胞结合。未检测到该抗体与一系列其他细胞系或有限数量的正常人组织有反应。外周血淋巴细胞、单核细胞、嗜碱性粒细胞、嗜酸性粒细胞、红细胞和血小板均不与该抗体反应。骨髓涂片显示NC-1与粒细胞系中早幼粒细胞及分化后期的髓细胞结合。当KG-1原始粒细胞样细胞系和U937髓单核细胞系暴露于能从碳水化合物残基上去除末端唾液酸的神经氨酸酶时，可诱导表达该抗原。同样，骨髓样本中的原始粒细胞在用神经氨酸酶处理后也能结合NC-1。用视黄酸处理诱导HL-60细胞分化为粒细胞后，其继续表达该抗原。当HL-60细胞被诱导分化为单核细胞时也观察到类似结果，尽管血液中的单核细胞不与该抗体结合。这些数据表明，抗体NC-1与分化早幼粒细胞阶段之后的髓细胞上表达的一种抗原发生反应。
