Yan Maokai, Jin Xingyue, Liu Yanhui, Chen Huihuang, Ye Tao, Hou Zhimin, Su Zhenxia, Chen Yingzhi, Aslam Mohammad, Qin Yuan, Niu Xiaoping
Guangxi Key Laboratory of Sugarcane Biology, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Agriculture, Guangxi University, Nanning, China.
Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Life Sciences, Fuzhou, China.
PeerJ. 2021 Oct 19;9:e12298. doi: 10.7717/peerj.12298. eCollection 2021.
Sugarcane ( L.), the major sugar and biofuel feedstock crop, is cultivated mainly by vegetative propagation worldwide due to the infertility of female reproductive organs resulting in the reduction of quality and output of sugar. Deciphering the gene expression profile during ovule development will improve our understanding of the complications underlying sexual reproduction in sugarcane. Optimal reference genes are essential for elucidating the expression pattern of a given gene by quantitative real-time PCR (qRT-PCR).
In this study, based on transcriptome data obtained from sugarcane ovule, eighteen candidate reference genes were identified, cloned, and their expression levels were evaluated across five developmental stages ovule (AC, MMC, Meiosis, Mitosis, and Mature).
Our results indicated that and were the most stably expressed genes during sugarcane female gametophyte development. Moreover, two genes, cell cycle-related genes and , were selected, and their feasibility was validated. This study provides important insights into the female gametophyte development of sugarcane and reports novel reference genes for gene expression research on sugarcane sexual reproduction.
甘蔗（L.）是主要的糖和生物燃料原料作物，由于雌性生殖器官不育导致糖的品质和产量下降，在全球范围内主要通过营养繁殖进行种植。解析胚珠发育过程中的基因表达谱将增进我们对甘蔗有性生殖潜在复杂性的理解。最佳内参基因对于通过定量实时PCR（qRT-PCR）阐明给定基因的表达模式至关重要。
在本研究中，基于从甘蔗胚珠获得的转录组数据，鉴定、克隆了18个候选内参基因，并评估了它们在胚珠的五个发育阶段（AC、MMC、减数分裂、有丝分裂和成熟）的表达水平。
我们的结果表明，和是甘蔗雌配子体发育过程中表达最稳定的基因。此外，选择了两个与细胞周期相关的基因和，并验证了它们的可行性。本研究为甘蔗雌配子体发育提供了重要见解，并报道了用于甘蔗有性生殖基因表达研究的新内参基因。
