The Affiliated Suzhou Science and Technology Town Hospital of Nanjing Medical University, Suzhou, China.
Suzhou Haike Medical Technology Co. Ltd., Suzhou, China.
Biomed Chromatogr. 2022 Mar;36(3):e5273. doi: 10.1002/bmc.5273. Epub 2021 Dec 8.
A simple and fast liquid chromatography-tandem mass spectrometry method was established and validated for the simultaneous determination of tenofovir alafenamide (TAF) and tenofovir (TNF) in human plasma. A simple protein precipitation procedure was employed to extract analytes from plasma. Chromatographic separation was performed on an Eclipse Plus C column utilizing a fast gradient elution starting with 2% of 2 mM ammonium acetate-formic acid (100/0.1, v/v) followed by increasing the percentage of acetonitrile. Detection was performed on a tandem mass spectrometer equipped with an electrospray ionization source operated in the positive ionization mode, using the transitions m/z 477.2 → m/z 346.1 for TAF and m/z 288.1 → m/z 176.1 for TNF. TAF-d and TNF-d were used as the internal standard of TAF and TNF, respectively. The method was validated in the concentration ranges 1.25-500 ng/mlfor TAF and 0.300-15.0 ng/ml for TNF with acceptable accuracy and precision.
建立并验证了一种用于同时测定人血浆中替诺福韦艾拉酚胺（TAF）和替诺福韦（TNF）的简单、快速的液相色谱-串联质谱法。采用简单的蛋白沉淀法从血浆中提取分析物。采用 Eclipse Plus C 柱进行色谱分离，以 2%的 2 mM 乙酸铵-甲酸（100/0.1，v/v）为起始，快速梯度洗脱，然后逐渐增加乙腈的比例。采用电喷雾电离源的串联质谱仪进行检测，正离子模式下操作，TAF 的监测离子对为 m/z 477.2→m/z 346.1，TNF 的监测离子对为 m/z 288.1→m/z 176.1。TAF-d 和 TNF-d 分别作为 TAF 和 TNF 的内标。该方法在 TAF 的浓度范围为 1.25-500ng/ml 和 TNF 的浓度范围为 0.300-15.0ng/ml 时具有可接受的准确度和精密度。
