Hou W, Wang Y Y, Zhang Y, Zhang L P, Xin G B, Qin S Y, Wang J F
Key Laboratory of Forensic Toxicology, Ministry of Public Security, Beijing Public Security Bureau, Beijing 100192, China.
School of Investigation, People' s Public Security University of China, Beijing 100038, China.
Fa Yi Xue Za Zhi. 2021 Aug;37(4):516-523. doi: 10.12116/j.issn.1004-5619.2021.310205.
Objective To establish a method combining QuEChERS and ultra-high liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） for rapid screening and testing of three types of new psychoactive tryptamines in human blood： 5-MeO-DALT, 5-MeO-MiPT and 5-MeO-DiPT. Methods The effects of the type of extractant, the type and dosage of salting-out agent, and the dosage of adsorbent on the test results of the three tryptamines were investigated. Blood samples were processed by QuEChERS method and then determined by UPLC-MS/MS. Results The linear relationships of 5-MeO-DALT, 5-MeO-MiPT and 5-MeO-DiPT in human blood were good in the range of 0.5-100, 0.5-100 and 0.2-100 ng/mL, respectively, with their coefficients higher than 0.99. The limits of detection （LODs） were 0.1-0.2 ng/mg. The recoveries ranged from 84.86% to 94.57%. Intra-day and inter-day precisions were good. Conclusion The method is simple, rapid, easy to operate and has a high recovery. It is suitable for the qualitative and quantitative study of tryptamines in blood and can provide the reference for public security organs to deal with related cases.
目的 建立一种结合QuEChERS和超高效液相色谱-串联质谱法（UPLC-MS/MS）快速筛查和检测人血液中三种新型精神活性色胺类物质：5-甲氧基-DALT、5-甲氧基-MiPT和5-甲氧基-DiPT的方法。方法 考察了萃取剂种类、盐析剂种类和用量、吸附剂用量对三种色胺类物质检测结果的影响。血液样本采用QuEChERS方法处理后，再用UPLC-MS/MS进行测定。结果 5-甲氧基-DALT、5-甲氧基-MiPT和5-甲氧基-DiPT在人血液中的线性关系良好，线性范围分别为0.5-100、0.5-100和0.2-100 ng/mL，相关系数均高于0.99。检测限（LOD）为0.1-0.2 ng/mg。回收率在84.86%至94.57%之间。日内和日间精密度良好。结论 该方法简便、快速、易操作，回收率高。适用于血液中色胺类物质的定性和定量研究，可为公安机关办理相关案件提供参考。
