Alexander-Sinclair E I, Okolov I N, Perepletchikova D A, Grechanaya Yu S, Zhurenkov K E, Blinova M I
Institute of Cytology of the Russian Academy of Sciences, Saint Petersburg, Russia.
Peter the Great St. Petersburg Polytechnic University, Saint Petersburg, Russia.
Vestn Oftalmol. 2021;137(5):86-92. doi: 10.17116/oftalma202113705186.
Effective and safe antiseptic eye preparations are necessary for prevention and treatment of infectious and inflammatory eye diseases.
evaluation of the effect of antiseptic eye drops on corneal and conjunctival epithelial cells.
Antiseptic eye drops «Bactavit», «Vitabact» and «Ocomistin» were the object of the study. Immortalized human corneal epithelial cell lines (HCE) and human conjunctiva (Chang Conjunctiva, Clone 1-5c-4) were used as the test systems. The viability of the cells was assessed by their metabolic activity and morphology using the MTT test and phase-contrast microscopy.
Antiseptic eye drops belonging to different groups of chemical compounds induced cytotoxic effects on the cells of corneal epithelium (HCE) and human conjunctiva (Chang Conjunctiva, Clone 1-5c-4) of varying degrees, leading to morphological and functional changes in those cells.
The study confirms the possibility of using cultured cells for the comparative assessment of the cytotoxic effect of antiseptic ophthalmic agents.
有效且安全的眼部抗菌制剂对于预防和治疗感染性及炎症性眼病是必要的。
评估抗菌眼药水对角膜和结膜上皮细胞的作用。
抗菌眼药水“Bactavit”、“Vitabact”和“Ocomistin”为研究对象。永生化人角膜上皮细胞系（HCE）和人结膜（张氏结膜，克隆1-5c-4）用作测试系统。使用MTT试验和相差显微镜通过细胞代谢活性和形态评估细胞活力。
属于不同化学化合物组的抗菌眼药水对角膜上皮（HCE）细胞和人结膜（张氏结膜，克隆1-5c-4）产生不同程度的细胞毒性作用，导致这些细胞出现形态和功能变化。
该研究证实了使用培养细胞对眼部抗菌剂的细胞毒性作用进行比较评估的可能性。
