Department of Plastic Surgery, Plastic Surgery Hospital.
Research Center, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 33# Badachu Road, Shijingshan District, Beijing, China.
J Craniofac Surg. 2021;32(8):2906-2911. doi: 10.1097/SCS.0000000000007902.
Reconstruction of craniofacial cartilage defects is among the most challenging operations in facial plastic surgery. The co-culture system of partial replacement of chondrocytes by stem cells has been confirmed effective in the repair of cartilaginous defects. The aim of this study is to compare chondrogenic properties of expanded adipose-derived stem cells (ADSCs) and stromal vascular fraction (SVF), including ADSCs/SVF monoculture and coculture with rabbit auricular chondrocytes (ACs). Analysis of morphology, histology, real-time polymerase chain reaction and glycosaminoglycans (GAG) quantification were performed to characterize the chondrogenesis of pellets. The triple differentiation potential of ADSCs had been confirmed. Further, using flow cytometry, the authors demonstrated that ADSCs and SVF have different characteristics in cell surface markers, and ADSCs are more enriched in cells from the mesenchymal lineage than SVF. GAG production of ADSCs is significantly higher than that of SVF in pellet monoculture, and pellet coculture of ADSCs and ACs are better in depositing cartilage matrix than the mixture of SVF and ACs. Our study suggests that ADSCs may be more suitable seed cells for craniofacial cartilage defect or deformity repair.
颅面软骨缺损的重建是面部整形外科中最具挑战性的手术之一。干细胞部分替代软骨细胞的共培养系统已被证实可有效修复软骨缺损。本研究旨在比较扩增的脂肪来源干细胞（ADSCs）和基质血管部分（SVF）的软骨生成特性，包括 ADSCs/SVF 单层培养和与兔耳软骨细胞（ACs）共培养。通过分析形态、组织学、实时聚合酶链反应和糖胺聚糖（GAG）定量来表征小球的软骨生成。已经证实 ADSCs 具有三重分化潜能。此外，通过流式细胞术，作者表明 ADSCs 和 SVF 在细胞表面标志物上具有不同的特征，并且 ADSCs 比 SVF 更富含间充质谱系的细胞。小球单层培养中 ADSCs 的 GAG 产量明显高于 SVF，ADSCs 和 ACs 的小球共培养比 SVF 和 ACs 的混合物更能沉积软骨基质。我们的研究表明，ADSCs 可能更适合用于颅面软骨缺损或畸形的修复。
