Tan S Y, Zou H, Yang C, Chen G, Chen S
School of Medicine, Hunan Normal University, Changsha 410000, China.
Beidaihe Sanatorium for Chinese Coal Miners, Qinhuangdao 066100, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2021 Oct 20;39(10):721-725. doi: 10.3760/cma.j.cn121094-20200601-00310.
To explore the effect of atractylenolide-1 (ATL-Ⅰ) on alveolar macrophages in silicosis patients. In December 2019, 12 male silicosis patients treated in Beidaihe Sanatorium for Chinese Coal Miners from July to September 2019 were selected by random sampling. Their alveolar macrophages were collected and divided into control group, ATL-Ⅰ group (100 μmol/L) and dimethyl sulfoxide (DMSO) group (100 μmol/L) . The exprossion levels of inflammatory factor interleukin-1β (IL-1β) , interleukin-6 (IL-6) , tumor necrosis factor α (TNF-α) were detected by enzyme-linked immunosorbent assay. The expression levels of autophagy associated protein microtubule associated protein light chain 3 (LC3) , autophagy substrate protein p62, lysosome associated membrane protein 2 (LAMP2) , apoptosis associated protein Cleaved caspase-3, nuclear factor kappa B (NF-κB) and its phosphorylated form (p-NF-κB) were detected by Western blot. Compared with the control group and DMSO group, the expression levels of IL-1β, IL-6, TNF-α in alveolar macrophages decreased significantly in the ATL-Ⅰ group (<0.05) , and the expression levels of p-NF-κB, the ratio of LC3-Ⅱ/LC3-Ⅰ also decreased significantly in the ATL-Ⅰ group (<0.05) . However, the expression levels of NF-κB, LAMP2, p62 and Cleaved caspase-3 in the ATL-Ⅰ group were not statistically different from those in the control group and DMSO group (>0.05) . There was no statistically significant differences in the expression of the above indexes between the control group and DMSO group (>0.05) . ATL-Ⅰ may reduce the release of inflammatory factors from alveolar macrophages and inhibit the activity of autophagy in silicosis patients, but it may not reduce the level of apoptosis.
探讨白术内酯-1（ATL-Ⅰ）对矽肺患者肺泡巨噬细胞的影响。2019年12月，采用随机抽样法选取2019年7月至9月在北戴河中国煤矿工人疗养院接受治疗的12例男性矽肺患者。收集其肺泡巨噬细胞，分为对照组、ATL-Ⅰ组（100μmol/L）和二甲基亚砜（DMSO）组（100μmol/L）。采用酶联免疫吸附测定法检测炎症因子白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、肿瘤坏死因子α（TNF-α）的表达水平。采用蛋白质免疫印迹法检测自噬相关蛋白微管相关蛋白轻链3（LC3）、自噬底物蛋白p62、溶酶体相关膜蛋白2（LAMP2）、凋亡相关蛋白裂解型半胱天冬酶-3、核因子κB（NF-κB）及其磷酸化形式（p-NF-κB）的表达水平。与对照组和DMSO组相比，ATL-Ⅰ组肺泡巨噬细胞中IL-1β、IL-6、TNF-α的表达水平显著降低（<0.05），ATL-Ⅰ组中p-NF-κB、LC3-Ⅱ/LC3-Ⅰ的比值也显著降低（<0.05）。然而，ATL-Ⅰ组中NF-κB、LAMP2、p62和裂解型半胱天冬酶-3的表达水平与对照组和DMSO组相比差异无统计学意义（>0.05）。对照组和DMSO组上述指标的表达差异无统计学意义（>0.05）。ATL-Ⅰ可能降低矽肺患者肺泡巨噬细胞炎症因子的释放并抑制自噬活性，但可能不会降低凋亡水平。
