College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.
The Affiliated Wuxi No. 2 People's Hospital of Nanjing Medical University, China.
J Immunol Methods. 2021 Dec;499:113179. doi: 10.1016/j.jim.2021.113179. Epub 2021 Oct 30.
In this study, a novel, simple, and rapid immunoassay for the determination of gastrin-17 (G-17) in human serum was established by combining immunomagnetic beads with time-resolved fluorescence immunoassay (TRFIA).
Immunomagnetic beads were coated with anti-G-17 M01 antibody, anti-G-17 M02 antibody was labeled with Eu chelates. The concentration of G-17 in the serum was detected with the double-antibody sandwich method.
The limit of background(LOB), limit of detection (LOD), and limit of quantification (LOQ) were 0.09, 0.104, and 0.39 pmol/L, respectively. The detection range of G-17-TRFIA was 0.39-100 pmol/L. The average intra- and inter-assay coefficients of variation (CV) were 5.95%-9.07% and 6.09%-8.14%, respectively. The recoveries for the serum samples ranged from 94.70% to 100.95%. The specificity of our G-17-TRFIA was acceptable. The correlation coefficient between G-17-TRFIA and commercial G-17-ELISA methods was R = 0.9092.
A novel G-17-TRFIA detection method was successfully established to provide a reference for the early diagnosis of patients with atrophic gastritis in clinical research.
本研究通过免疫磁珠与时间分辨荧光免疫分析（TRFIA）相结合，建立了一种用于检测人血清中胃泌素-17（G-17）的新型、简单、快速的免疫分析方法。
免疫磁珠包被抗 G-17 M01 抗体，抗 G-17 M02 抗体用 Eu 螯合物标记。采用双抗体夹心法检测血清中 G-17 的浓度。
本方法的背景限（LOB）、检测限（LOD）和定量限（LOQ）分别为 0.09、0.104 和 0.39 pmol/L。G-17-TRFIA 的检测范围为 0.39-100 pmol/L。批内和批间变异系数（CV）的平均值分别为 5.95%-9.07%和 6.09%-8.14%。血清样本的回收率在 94.70%-100.95%之间。该 G-17-TRFIA 的特异性可接受。与商业 G-17-ELISA 方法的相关系数为 R=0.9092。
成功建立了一种新型的 G-17-TRFIA 检测方法，为临床研究中萎缩性胃炎患者的早期诊断提供了参考。
