Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan.
Meguro Parasitological Museum, Meguro-ku, Tokyo 153-0064, Japan.
Parasitol Int. 2022 Apr;87:102491. doi: 10.1016/j.parint.2021.102491. Epub 2021 Oct 30.
Pulsating broodsacs of Leucochloridium sp. (Trematoda: Leucochloridiidae) were found from amber snails (Succinea lauta) in Iwate, the northern part of Honshu, Japan. A pattern with red-brown vertical stripes was characteristic of the broodsac. Very similar broodsacs were already detected from Okinawa Islands, the southern archipelago of Japan, and tentatively identified as Leucochloridium cf. passeri. A phylogenetic analysis based on DNA sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) showed that Leucochloridium sp. is different at species level from L. cf. passeri and that both species are related to Leucochloridium vogtianum from Europe. In this study the definitive identification of larval Leucochloridium sp. was impossible, but the resulting phylogeny confirmed that at least 4 species of Leucochloridium are distributed in Japan, depending on locality and climate. The DNA barcode generated in this study will be useful in detecting the adult stage of Leucochloridium sp. from birds.
在日本本州北部岩手县的琥珀蜗牛（Succinea lauta）中发现了旋口科（Leucochloridiidae）Leucochloridium sp. 的脉动育囊。育囊的特征是具有红棕色垂直条纹的图案。在日本的南部群岛冲绳岛已经发现了非常相似的育囊，并初步鉴定为 Leucochloridium cf. passeri。基于线粒体细胞色素 c 氧化酶亚基 1（cox1）的 DNA 序列的系统发育分析表明，Leucochloridium sp. 在种的水平上与 Leucochloridium cf. passeri 不同，并且这两个物种与欧洲的 Leucochloridium vogtianum 有关。在这项研究中，幼虫 Leucochloridium sp. 的明确鉴定是不可能的，但产生的系统发育证实，至少有 4 种 Leucochloridium 分布在日本，这取决于地理位置和气候。本研究中生成的 DNA 条码将有助于从鸟类中检测到 Leucochloridium sp. 的成虫阶段。
