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新西兰产酸米粉中优势乳酸菌和酵母菌的鉴定

Identification of dominant lactic acid bacteria and yeast in rice sourdough produced in New Zealand.

作者信息

Yang Qiwei, Rutherfurd-Markwick Kay, Mutukumira Anthony N

机构信息

School of Food and Advanced Technology, Massey University, Auckland, 0745, New Zealand.

School of Health Sciences, College of Health, Massey University, Auckland, 0745, New Zealand.

出版信息

Curr Res Food Sci. 2021 Oct 15;4:729-736. doi: 10.1016/j.crfs.2021.10.002. eCollection 2021.

Abstract

This study characterised a commercial New Zealand gluten free (GF) rice sourdough and its starter culture composition. Acidity of the mother sourdough, dough before proofing and dough after proofing was determined during the production of rice sourdough bread, and colour was measured for the baked bread. Yeast and lactic acid bacteria (LAB) were enumerated in the rice sourdough samples and representative colonies characterised using API kits and sequenced by the Internal Transcribed Spacer and 16 S rRNA region. Sourdough LAB isolates were identified as DSM 10667 and CIP 102980 and the yeast isolates as CBS 1171. Dough acidity increased significantly (p < 0.05) during fermentation due to the metabolic activities of the sourdough cultures. After baking, the colour of the rice sourdough bread crust was similar to that of unleavened wheat bread (golden brown). The improved colour of the rice sourdough bread crust may be a result of combined use of sourdough technique and optimal baking conditions. The results of this study may allow bakers to improve the overall quality of GF rice sourdough baked bread by selecting suitable fermentation and baking parameters.

摘要

本研究对一种商业化的新西兰无麸质(GF)米制酸面团及其发酵剂培养物成分进行了表征。在米制酸面团面包的制作过程中,测定了母酸面团、醒发前面团和醒发后面团的酸度,并对烘焙面包的颜色进行了测量。对米制酸面团样品中的酵母和乳酸菌(LAB)进行了计数,并使用API试剂盒对代表性菌落进行了表征,并通过内转录间隔区和16S rRNA区域进行了测序。酸面团LAB分离株被鉴定为DSM 10667和CIP 102980,酵母分离株被鉴定为CBS 1171。由于酸面团培养物的代谢活动,面团酸度在发酵过程中显著增加(p < 0.05)。烘焙后,米制酸面团面包皮的颜色与未发酵的小麦面包(金棕色)相似。米制酸面团面包皮颜色的改善可能是酸面团技术和最佳烘焙条件联合使用的结果。本研究结果可能使面包师通过选择合适的发酵和烘焙参数来提高无麸质米制酸面团烘焙面包的整体质量。

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