BCCM/IHEM, Mycology and Aerobiology, Sciensano, Bruxelles, Belgium.
InBioS, Evolution and Conservation Biology, University of Liège, Liège, Belgium.
Microb Genom. 2021 Nov;7(11). doi: 10.1099/mgen.0.000707.
The medically relevant species complex has a variety of phenotypic presentations but shows relatively little genetic differences. Conventional barcodes, such as the internal transcribed spacer (ITS) region or the beta-tubulin gene, are not able to completely resolve the relationships between these closely related taxa. , and are currently accepted as separate species. However, the status of certain variants, including the morphotypes and and the morphotype , remains to be deciphered. We conducted the first phylogenomic analysis of the species complex by studying 3105 core genes of 18 new strains from the BCCM/IHEM culture collection and nine publicly available genomes. Our analyses revealed a highly resolved phylogenomic tree with six separate clades. , and were confirmed in their status of species. The morphotypes and all grouped in their own respective clade with high support, suggesting that these morphotypes should be reinstituted to the species-level. Robinson-Foulds distance analyses showed that a combination of two markers (a ubiquitin-protein transferase and a MYB DNA-binding domain-containing protein) can mirror the phylogeny obtained using genomic data, and thus represent potential new markers to accurately distinguish the species belonging to the complex.
该医学相关种复合体具有多种表型表现，但遗传差异相对较小。传统的条形码，如内转录间隔区（ITS）区域或β-微管蛋白基因，无法完全解决这些密切相关分类群之间的关系。目前， 、 和 被认为是独立的物种。然而，某些变体的地位，包括 形态型 和 以及 形态型 ，仍有待破译。我们通过研究来自 BCCM/IHEM 培养物收集的 18 个新菌株和 9 个公开可用基因组的 3105 个核心基因，对 种复合体进行了首次系统基因组分析。我们的分析揭示了一个高度解析的系统基因组树，有六个独立的分支。 、 和 被确认为物种。形态型 和 都在自己的分支中得到了高度支持，这表明这些形态型应该重新确立为物种水平。罗宾逊-福尔德斯距离分析表明，两个标记（泛素-蛋白转移酶和 MYB DNA 结合域蛋白）的组合可以反映使用基因组数据获得的系统发育，因此代表了潜在的新标记，可以准确地区分属于 复合体的物种。
